RP-HPLC法测定苦皮藤素中苦皮素A的含量  

Determination of Angulatin A in Angulatin by RP-HPLC

作　　者：卢桂荣[1] 吴鸣建[1] 赵天增[2] 刘金盾[1] 

机构地区：[1]郑州大学化工学院,郑州450002 [2]河南省科学院天然产物重点实验室,郑州450002

出　　处：《河南科学》2007年第3期395-397,共3页Henan Science

基　　金：河南省自然科学基金项目(511022800)

摘　　要：建立了一种测定苦皮藤根皮及其产品中苦皮素A的反相高效液相色谱方法.采用Zorbax eclipse XDB C18(150 mm×4.6 mm i.d.,5μm)色谱柱,以甲醇-水(V甲醇∶V水=70∶30)为流动相,流速为1.0 ml/min,在波长232 nm处进行检测.实验结果表明,在0.000 9～0.020 7 mg/ml范围内,苦皮素A的峰面积与质量浓度呈良好的线性关系,相关系数R=0.999 5,平均回收率为98.8%,相对标准偏差RSD=0.313%.该法简便、快速且重现性好,适用于苦皮藤原料及其产品中苦皮素A的定量分析.A method for the determination of angulatin A in root barks and the products of Celastrus angulatus by reverse-phase high performance liquid chromatography was developed. Zorbax eclipse XDB C18（150 mm×4.6 mm i.d., 5 μm）was chosen as analytical column, methanol-water （70：30, V：V） at a flow-rate of 1.0 ml/min as mobile phase with UV detection at 232 nm. Results of experiments prove that the relationship between peak area and concentration of angulatin A in the range from 0.000 9 to 0.020 7 mg/ml showed a good linearity,the correlation coefficient was 0.999 5, the average recovery was 98.8 %, and relative standard derivation （RSD） was 0.313 %. The method is simple, rapid and reproducible, and suitable for quantitative analysis of angulatin A in the materials and products of Celastrus angulatus.

关 键 词：反相高效液相色谱法 苦皮素A 苦皮藤素 苦皮藤 

分 类 号：O629.9[理学—有机化学] S482.1[理学—化学]