贵农775抗条锈病基因标记的相关片段克隆及荧光原位杂交  

Correlation Segment Clone of Molecular Markers and Fluorescence in Situ Hybridization for Stripe Rust Resistance Gene in Wheat Germplasm Guinong775

作　　者：葛昌斌 徐如宏[2] 郭春强 廖平安 张庆勤[2] 

机构地区：[1]河南省漯河市农业科学院,河南漯河462000 [2]贵州大学麦作研究中心,贵阳550025

出　　处：《种子》2007年第5期40-43,共4页Seed

基　　金：贵州省省长专项基金(2004-2007)资助

摘　　要：小麦抗病种质贵农775是原贵州大学张庆勤教授利用远缘杂交育成的著名抗源材料,在抗病育种和生产上具有重要的应用价值,本研究利用分子标记的相关片段克隆和荧光原位杂交技术对其进行了研究,将贵农775中与Y rGA连锁的标记片段克隆、测序,用荧光原位杂交技术鉴定,贵农775为簇毛麦的新的易位系,用特异引物S 2018扩增作探针,与贵农775体细胞中期染色体杂交,进一步证明了与其连锁的Y rGA基因也是来自簇毛麦。Guinong 775 is a famous germplasm, which derived from Hayaldia Villosa hybriding with eummer wheat and common wheat cultivar by professor Zhang in Guizhong University, and it is important in wheat breeding and production practice. This article studied for the usage of fluorescent in situ hybridization and Correlation Segment Clone of Molecular Markers to Guinong 775,The results indicated that the Guinong 775 was not a wheat- Haynaldia villosa segment translocation line, and it is different to the research results of Liuda Jun et al. The results of fluorescence in situ hybridization（FISH） using genomic DNA of Haynaldia viIlosa and the special probe of S 2018 as probe indicated that the Guinong 775 was a wheat- Haynaldia villosa new seg- ment translocation line. It indicated that the YrGA comes from Haynaldia villosa again.

关 键 词：贵农775 抗条锈病基因 荧光原位杂交 簇毛麦 克隆 

分 类 号：S512.1[农业科学—作物学]