大豆植物体中铝形态的^(27)AlNMR分析  

Form Analysis of Aluminum in Soybean by ^(27)AlNMR

作　　者：郑人卫[1] 刘鹏[1] 陆霞梅[1] 徐根娣[1] 蔡妙珍[1] 

机构地区：[1]浙江师范大学植物学实验室,浙江金华321004

出　　处：《华南理工大学学报（自然科学版）》2007年第4期112-116,共5页Journal of South China University of Technology(Natural Science Edition)

基　　金：国家自然科学基金资助项目(3054005640573052);浙江省自然科学基金资助项目(303461504135)

摘　　要：铝毒是酸性土壤中影响植物生长的最重要的因素,对生物样品中铝的研究有助于了解铝在植物中的形态与运输.文中以华春18大豆(Glycine max)为研究材料,首次利用无破坏性、精密度高的27AlNMR对大豆根、茎、叶部铝的形态进行了分析.结果表明:铝在大豆根部以铝-苹果酸(1∶3)复合物和铝-柠檬酸(1∶1)复合物两种形态存在,其化学位移分别在11.16和-0.38左右;在茎和叶中没有检测到铝-有机酸复合物;大豆根茎叶中的可溶性铝主要以[AlO4Al12(OH)24(H2O)12]的形态存在,其化学位移在63.2左右.Aluminum toxicity is known as an important factor that affects the growth of crops in acid soil. The investigation into the Al in biological samples is helpful to reveal the form and transportation of Al in crops. In this paper, the nondestructive ^27AlNMR with high accuracy is adopted to analyze the forms of A1 in the roots, stems and leaves of soybean （Glycine max） cv Huanchun No. 18. The results show that （1） the Al in soybean roots is mainly chelated to malate and citrate in the forms of Al-malate （ 1 ： 3 ） and Al-citrate （ 1： 1 ） complexes, whose chemical shifts are respectively 11.16 and -0.38, （2） no Al-organic acid complexes are found in soybean stems or leaves, and （3） the dissoluble Al in soybean roots, stems and leaves mainly exists in the form of [ AlO4Al12 （OH）24 （H/O）12 ], whose chemical shift is 63.2.

关 键 词：铝 大豆 ^27Al核磁共振 形态 有机酸复合物 

分 类 号：O657.61[理学—分析化学] Q945.12[理学—化学]