绵羊卵母细胞孤雌发育的研究  

Study on Parthenogenetic Development of Ovine Oocytes

作　　者：潘晓燕[1] 杨梅[2] 王正朝[1] 汪立琴[2] 陈童[2] 赵雪萍[1] 郭志勤[2] 王锋[1] 

机构地区：[1]南京农业大学动物胚胎工程技术中心,江苏南京210095 [2]新疆畜牧科学院农业部家畜繁育生物技术重点开放实验室,新疆乌鲁木齐830000

出　　处：《西北农业学报》2007年第3期7-10,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：农业部农业结构调整重大技术研究专项(05-07-01B)

摘　　要：系统地研究了绵羊卵母细胞成熟培养时间、6-DMAP激活时间以及改进的培养液对绵羊卵母细胞孤雌发育的影响。结果表明:卵母细胞成熟培养25 h和26 h激活组的囊胚率和孵化率显著高于成熟培养24 h激活组;6-DMAP激活液作用2 h,激活效果较好;培养液改进后,孤雌胚的囊胚率有上升趋势,但差异不显著。在本试验条件下,卵母细胞体外成熟培养25 h后在6-DMAP中激活2 h,利用改进后的培养液进行培养,能获得较好的发育效果。The paper systematically studied the effect of time of maturation in vitro , duration of 6- DMAP treatment and culture medium system on development of parthenogenetic ovine embryos. The results showed that oocytes matured for 25 h or 26 h before parthenogenetic development resulted higher blastocyst and hatched blastocyst rate than that matured for 24 h（P〈 0.01）;the better activation rate was obtained when oocytes were treated with 6-DMAP for 2 hr;the development rate of blastocyst would tend to arise in new culture medium though there was no significant difference. These results suggested that to obtain better activation and development ,oocytes matured for 25 h should be activated by 6-DMAP treatment for 2 hr and cultured in new culture medium.

关 键 词：绵羊 卵母细胞 孤雌激活 6-DMAP 

分 类 号：S826[农业科学—畜牧学]