色氨酸代谢在再生障碍性贫血中的实验研究  

Experimental study on tryptophan catabolism in aplastic anemia

作　　者：周琨[1] 孙汉英[1] 戴敏[1] 徐惠珍[1] 刘文励[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院血液科,武汉430030

出　　处：《国际输血及血液学杂志》2007年第3期214-217,共4页International Journal of Blood Transfusion and Hematology

摘　　要：目的 探讨色氨酸代谢参与再生障碍性贫血（Aplastic Anemia，AA）的免疫系统异常活化的发病机制。方法 建立免疫介导骨髓衰竭模型，用反向高效液相色谱法检测色氨酸（Trp）和其代谢产物犬尿氨酸（Kyn）的血清水平，以及Trp的限速酶——吲哚胺双加氧酶（IDO）活性，用RT—PCR方法检测骨髓单个核细胞IDOmRNA的表达水平，探讨色氨酸在疾病进程中的异常表现。结果 模型小鼠Trp浓度明显低于正常小鼠（P〈0．05），模型小鼠IDO活性明显高于正常小鼠（P〈0.05），模型小鼠的单个核细胞IDOmRNA表达水平较正常小鼠高（P〈0．05）。结论 免疫介导骨髓衰竭模型小鼠的色氨酸限速酶IDO的表达水平和活性均较正常小鼠高，免疫系统的异常活化导致必需氨基酸色氨酸的不足。Objective To observe the changes of tryptophan catabolism in aplastic anemia and to analyze the relationship between the changes of tryptophan catabolism and the expression of IDO. Methods A mouse model of lymphocyte infusion-induced bone marrow failure was developed. High-performance liquid chromatography （HPLC） technique was used to detect the concentration of tryptophan and kynurenine in serum and IDO activity. The expression of IDO mRNA was detected with RT-PCR. Results The IDO mRNA expression and IDO activity in mouse model was higher than control, with significant difference （P 〈 0. 05）, while serum tryptophan concentration was lower than control significantly. Conclution The deficiency of the essential aminoacid tryptophan in aplastic anemia mostly results from immune activation involved in the pathogenesis of the disease. IDO expression level and tryptophan concentration are important for pathogenesis and treatment strategy of aquired Aplastic Anemia.

关 键 词：获得性再生障碍性贫血 吲哚胺双加氧酶 色氨酸 

分 类 号：R5[医药卫生—内科学]