6O％氧暴露对早产大鼠肺血管内皮生长因子及其受体表达的影响  

Effects of 60 % oxygen exposure on the expression of vascular endothelial growth factor and its receptor in lungs of preterm rats

作　　者：王伟[1] 韦伟[1] 王安茹[1] 蒙晶[1] 宁琴[1] 罗小平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院儿科学系,武汉430030

出　　处：《中华围产医学杂志》2007年第3期186-189,I0001,共5页Chinese Journal of Perinatal Medicine

基　　金：国家杰出青年科学基金（30125019）;国家重点基础研究发展计划（973计划）（2005CB522507）

摘　　要：目的观察60％氧暴露对早产大鼠肺血管内皮生长因子（vascular endothelial growthfactor，VEGF）及其受体——胎肝激酶-1受体（fetal liver kinase-1，Flk-1）表达的影响，探讨其与新型支气管肺发育不良发病机制之间的关系。方法早产鼠生后6h内随机分为中浓度氧组（简称中氧组）和空气组，空气组置于常压空气中，中氧组置于氧体积分数为60％的氧舱中，两组动物均于生后1、4、7、11和14d各随机取8只，行HE染色，观察肺组织形态学结构，做辐射状肺泡计数（radial alveolar counts，RAC）；采用Western印迹和RT-PCR技术检测各组肺组织VEGF及其受体Flk-1蛋白及mRNA表达水平。结果（1）中氧组RAC在7d时为8．32±0．11，11d时为8．53±0．08，14d时为9．03±0．17，明显高于空气组相应时间点的RAC值；（2）中氧组VEGF及其受体Flk-1 mRNA表达水平在第7、11和14天均低于空气组相应时间点，差异有统计学意义（P〈0．05）；（3）中氧组VEGF蛋白表达水平在第11、14天时低于空气组；受体Flk-1蛋白表达水平与其mRNA表达变化规律基本一致。结论60％氧暴露可引起早产大鼠肺部VEGF及其受体Flk-1表达下降，可能是引起肺微血管发育障碍及肺泡化进程受阻，进而导致新型BPD发生的重要因素。Objective To investigate the changes of VEGF and its receptor, fetal liver kinase-1 （Flk-1） expression, in lungs of premature rats exposed to 60% oxygen and elucidate the relationship between O2 exposure and the pathogenesis of bronchopulmonary dysplasia （BPD）. Methods Midoxygen lung injury model was established by exposing to 60% O2 in the preterm period of Spraque Dawley rats. Rats exposed to air were used as control group. At day 1, 4, 7, 11 and 14 after birth, the lungs from each subjects were removed and dissected from the main bronchi for analysis. Eight rats of each group were used to assess lung histologic changes with HE staining. Lung VEGF and its receptor Flk-1 mRNA and protein levels were measured by reverse transcription polymerase chain reaction （RT-PCR） and western blot. Results （1） Radial alveolar counts in mid-oxygen group was 8.23±0.11 at day 7, 8. 53±0.08 at day 11 and 9. 03±0.17 at day 14, lower than control group（P〈 0. 05）. （2）VEGF mRNA, Flk-1 mRNA and protein level in mid-oxygen group were lower than control group at day 7, 11 and 14 （P〈0.05）. （3） The VEGF164 protein levels in mid-oxygen group were found to decrease at day 11 and 14 comparing with control group （P〈0.05）. Conclusions Changes of the expression of VEGF and its receptor Flk-1 in lungs of premature rats exposed to 60% oxygen may contribute to the development of BPD.

关 键 词：高氧症 支气管肺发育不良 血管内皮生长因子A 血管内皮生长因子受体2 大鼠 婴儿 早产 

分 类 号：R722[医药卫生—儿科]