不同聚合度琼寡糖对肝细胞内外抗氧化活性的影响  

AGARO-OLIGOSACCHARIDE ENHANCES THE ANTIOXIDANT PROPERTY OF LIVER CELL IN VITRO AND IN VIVO

作　　者：陈海敏[1] 马红辉[1] 郑立[2] 林伟[3] 严小军[1] 

机构地区：[1]宁波大学海洋生物重点实验室,宁波315211 [2]国家海洋局第一研究所,青岛266061 [3]中国科学院海洋研究所,青岛266071

出　　处：《海洋与湖沼》2007年第3期253-258,共6页Oceanologia Et Limnologia Sinica

基　　金：浙江省高校青年教师资助计划项目;2005.09-2007.09;浙江省自然科学基金资助项目;Y506132号;浙江省教育厅资助项目;20051693号。

摘　　要：通过研究不同聚合度琼寡糖对肝细胞的内外抗氧化活性的影响,对琼寡糖聚合度与活性间的关系进行了评价。利用二苯基苦味酰肼自由基(DPPH·)检测方法研究了琼寡糖的细胞外抗氧化活性,结果表明,琼六糖具较高的淬灭自由基活性的能力。采用二氯荧光素检测方法(DCF)细胞内的活性,结果与细胞外的活性结果相吻合,并抑制H2O2所造成的细胞氧化损伤及死亡。通过选用五种聚合度的琼胶寡糖,研究了其抗氧化活性,从细胞内、外两个水平说明琼六糖均有良好的清除自由基活性的能力,进一步证实琼寡糖的抗氧化作用较强。The extracellular and intracellular antioxidant activities of agaro-oligosaccharide in different degrees of polymerizations （DPs） were evaluated in this paper with the establishment of a relationship between the activity and DPs. The extracellular antioxidant capability of oligosaccharides was determined by examining 1,1-dipheny1-2-picryl-hydrazyl （DPPH.） radical scavenging activity. Dichlorofluorescein （DCF） assay was used to evaluate the influence of DP of agaro-oligosaccharide on antioxidant capability in normal liver cell system （L-02）, which was directly oxidized by exposing to H2O2. The cellular cytotoxic impact of H2O2 and agaro-oligosaccharide on liver cell L-02 was also assessed with MTT assay. The DPPH. radical scavenging assay indicated that agarohexaose at 1.85 mg/ml concentration showed higher DPPH-scavenging capability in IC50 than agarooctaose at 2.62 mg/ml. Both agarobiose and agarotetraose showed relatively low scavenging activity at lower concentration below 8 mg/ml. Intracellular test showed that DPs of agaro-oligosaccharides could affect the antioxidation level significantly. Among 5 agaro-oligosaccharides （agarobiose, agarotetraose, agarohexaose, agarooctaose and agarodecaose）, agarohexaose had the strongest protection against H2O2-induced ROS, and the ROS yield was decreased by half. To exclude the possibility of cellular cytotoxic property of agaro-oligosaccharides, a cell viability assay was carried out without H2O2 treatment. The result shows that agaro-oligosaccharides exhibited no cytotoxic effects on human liver cell L-02. In contrast, the oligosaccharides especially the agarobiose could stimulate the growth of liver cells. The liver cell viability exposed to H2O2 was as low as 60%. However, by pre-treatment with the 5 agaro-oligosaccharides, the viability was enhanced obviously. Therefore, agaro-oligosaccharides especially agarohexaose are potential antioxidants to protect cells damage caused by reactive oxygen species, exhibiting desirable effects on intercellular

关 键 词：琼寡糖 抗氧化 DPPH DCF 肝细胞 

分 类 号：Q53[生物学—生物化学]