酒石酸钾-甘油密度梯度离心法纯化对虾肝胰腺细小病毒的方法学研究  

METHODOLOGICAL STUDY OF THE PURIFICATION OF THE HEPATOPANCREATIC PARVOVIRUS OF PENAEID SHRIMP BY A POTASSIUM TARTRATE GLYCEROL DENSITY GRADIENT CENTRIFUGATION

作　　者：薛清刚[1] 罗挽涛[1] 宋庆云[1] 王文兴[1] 杨虹[1] 

机构地区：[1]国家海洋局第一海洋研究所

出　　处：《水产学报》1997年第1期1-5,共5页Journal of Fisheries of China

摘　　要：阐述一种从对虾肝胰腺组织中纯化肝腺细小病毒的方法。将对虾肝胰腺组织样品研磨并进行适当脱脂处理后，进行一系列的浓缩和过３５％（ｗ／ｗ）蔗糖垫离心，然后通过一个不连续的酒石酸钾－甘油密度梯度离心纯化。结果证实，该方法对样品中的病毒颗粒的分离和纯化效果均较理想，可以满足有关研究工作的需要。经与ＣｓＣｌ密度梯度离心法比较，提示两者效果相似。A method to purify the hepatopancreatic parvovirus of penaeid shrimp from the infected tissue by density gradient centrifugation using potassium tartrate glycerol as substrate is described in this article. Taken from the heavily infected shrimp population, the hepatopancrea was ground thoroughly and treated with 1,1,2 trichlorotrifuoroethane or chloroform. After the concentration with 6%～8% of polyethylene glycol (PEG), centrifugation through a sucrose cushion of 35% (w/w) was performed. Then, the resulted pellet was resuspended and centifugalized through a density gradient of potassium tartrate glycerol prepared according to Ashley (1982). It is indicated from the purification result that the method is as effective as the CsCl density gradient centrifugation yet less expensive.

关 键 词：酒石酸钾 对虾 肝胰腺细小病毒 纯化 

分 类 号：S945.26[农业科学—水产养殖]