土霉素在鳗鲡组织中残留的消除规律  

STUDIES ON THE DEPLETION OF OXYTETRACYCLINE RESIDUES ON EEL(ANGUILLA JAPONICA) TISSUES

作　　者：李美同[1] 郭文 林仲锋 郑国兴[2] 沈亚林[2] 周凯[2] 王蓓 金凤 朱鑫源 

机构地区：[1]中国兽药监察所 [2]中国水产科学研究院东海水产研究所 [3]上海兽药监察所

出　　处：《水产学报》1997年第1期39-43,共5页Journal of Fisheries of China

摘　　要：在２３～２７℃水温中以每天２００ｍｇ／ｋｇｂ．ｗ．剂量灌服土霉素五天，测定鳗鲡组织中土霉素残留的消除规律。停药后每天剖取五条鱼的肉和肝，用４％三氯乙酸－０．０２ｍｏｌ／Ｌ磷酸氢二钠水溶液和三氯甲烷匀浆，用石油醚除去脂肪。水层用固相萃取Ｃ１８柱净化后，用ＨＰＬＣ法在３６０ｎｍ下测定土霉素残留。色谱柱：ＯＤＳ－５μ２５０×４．０ｍｍ。流动相：甲醇＋乙腈＋二甲基甲酰胺＋ＥＤＴＡ－柠檬酸钠缓冲液＝１＋１．２＋０．４２＋１．９。停药１天鱼肉中土霉素含量达到１．０ｍｇ／ｋｇ，停药７天降低至０．Eels were kept in experimental tanks with aerated tapwater at temperatures of 23～27℃. The weight per fish was 200～300g.The eels were fed medicated feed by syringe. The dosage of Oxytetracycline (OTC) was 200mg/kg b.w.per day for 5 days. Muscle and liver tissues were taken from five eels at hours 0, 6, 12, 24,48, 72, 120, 168, 216 after the last dose with OTC. Tissues samples were homogenized with 4% trichloroacetic acid-0.02mol/LNa 2HPO 4 aqueous solution and trichloromethane, fat was removed by petroleum ether. The aqueous layer was purified by a C 18 solid phase extraction column.The OTC residues were determined by HPLC external standard method using a SGE-ODS-5 μ 250×4.0mmID column with a mobile phase of methanol acetonitrile DMF buffer (Na 2EDTA citric acid sodium citrate water)=1∶1.2∶0.42∶1.9 at UV detectionwavelength of 360nm. The OTC level in eel muscle reached 1.0mg/kg at 24 hours and decreased to 0.1mg/kg at 168 hours (7 days)after withdrawal.

关 键 词：鳗鲡 土霉素 残留 

分 类 号：S965.223[农业科学—水产养殖] S948[农业科学—水产科学]