马尾松幼树根系固氮细菌的发现及其初步研究  

THE DISCOVERY AND PRELIMINARY STUDY ON NITROGEN-FIXING BACTERIA FROM THE ROOTS OF MASSON PINE SAPLINGS

作　　者：谢莉华[1] 周建[1] 周政贤[1] 

机构地区：[1]贵州农学院造林生态研究室

出　　处：《林业科学》1997年第2期152-160,共9页Scientia Silvae Sinicae

基　　金：国家"八五"重点科技攻关课题

摘　　要：对六类不同岩性发育的土壤中马尾松幼树根系的固氮细菌进行了初步研究，结果发现，在马尾松幼树的根系上能普遍分离到固氮细菌，但在不同岩性的土壤中分离的效果有差异；主、侧根及树势等的不同都影响分离效果。采用根段法和富集法共分离到１４２个菌株。其中一株经鉴定为争论产碱菌（Ａｌｃａｌｉｇｅｎｅｓｐａｒａｄｏｘｕｓ）。并对其中２６个菌株进行了回接实验，有９个菌株回接成功。固氮效能的测定结果表明其中Ａ｀１０、Ａ｀１３、Ｇ１、ｇ２ｗ四株菌的固氮效能为每消耗１ｇ葡萄糖分别固定氮２．１０—３．５０ｍｇ、４．２０—５．１３ｍｇ、３．６６—６．８３ｍｇ和１．９３—２．６０ｍｇ。This paper presents the preliminary study on nitrogen-fixing bacteria from the roots of Masson pine saplings.The results show that nitrogen-fixing bacteria can be isolated universally from the roots of masson pine saplings planted in six kinds of soils,but the effects of isolation among six kinds of soils were different.142 strains of the nitrogen-fixing bacteria were obtained by two methods,of which one strain A`10 is identified as Alcaligenes paradoxus.Inoculation of seeds of masson pine with twenty-six strains were done,while inoculation of nine strains were successful.Nitrogenase activity of four strains,i.e.A`10,A`13,G1 & g2w,were measured,and the results are as following:A`10,2.10—3.50 mgN/g glucose;A`13,4.20—5.13 mgN/g glucose;G1,3.66—6.83 mgN/g glucose;g2w,1.93—2.60 mgN/g glucose.

关 键 词：马尾松 固氮细菌 固氮效能 

分 类 号：S791.248[农业科学—林木遗传育种] S718.83[农业科学—林学]