棉子糖低钾右旋糖酐液供体肺灌注保存的临床病理学研究  

Lung donor perfused with raffinose-low-potassium dextran solution for lung transplantation:A clinicopathological study

作　　者：杨国仪 陈静瑜 夏钰弘 虞敏红 洪建刚 吴波[2] 姜少军[2] 周晓军[2] 

机构地区：[1]无锡市胸科医院肺移植中心病理科,无锡214073 [2]南京军区南京总医院病理科,南京210002

出　　处：《临床与实验病理学杂志》2007年第2期210-212,共3页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的 探讨棉子糖低钾右旋糖酐液（raffinose-low-potassium dextran solution,R-LPD液）供肺灌注低温保存后的组织形态学变化。方法 应用光镜、电镜和免疫组化技术对R-LPD液灌注的18例肺移植供肺标本按不同的时间段进行观察。结果 R-LPD供肺灌注低温保存的最佳时间是6-8h,随着保存时间延长,供体肺的形态结构改变也逐渐明显,保存30h时,可见肺泡壁及血管周围间质组织水肿,肺泡间隔断裂,部分肺泡上皮细胞变性、坏死、脱落。结论 R-LPD液在肺移植冷缺血保存期中起较好的肺泡上皮细胞保护作用,比低钾右旋糖酐液（low-potassium dextran solution,LPD液）具有明显的优越性。Purpose To observe the change of the structure of the morphology in lung perfused with raffinose-low-potassium dextran solution （ R-LPD＇ S）. Methods The human lung perfused with R-LPD＇ S was observed under light microscope and electron microscope and analyzed with immunohistochemistry in 18 cases. Results The best time of preservation in lung perfused with R-LPDS was 6 - 8 hours. Morphologic changes of the structure in the tissue cells were gradually obvious with time. When 30 hours of preservation the tissue of donor lung perfused with R-LPDS showed a decreased number of intact type Ⅱ type pneumocytes and a few damaged cells or cellular necrosis. Minimal interstitial and alveolar edema with interstitial macrophage infiltration was also observed. Conclusions R-LPD＇ S exerts a cytoprotective effect on preservation of donor lung during pulmonary grafts, and it is superior to low-potassium dextran solution for this purpose.

关 键 词：肺移植 器官保存液 棉子糖 右旋糖酐类 免疫组织化学 超微结构 

分 类 号：R322-34[医药卫生—人体解剖和组织胚胎学] R655.3[医药卫生—基础医学]