霍山石斛不同生长阶段遗传稳定性的RAPD分析  

Analysis on genetic stability in different development stages of Dendrobium huoshanense by RAPD

作　　者：刘石泉[1] 李小军[2] 余庆波[2] 谢红[2] 周根余[2] 

机构地区：[1]湖南城市学院化学与环境工程系,湖南益阳413000 [2]上海师范大学生命与环境科学学院生物系遗传学实验室,上海200234

出　　处：《中国中药杂志》2007年第10期902-905,共4页China Journal of Chinese Materia Medica

基　　金：湖南省高等学校科学研究项目(06C218)

摘　　要：目的：研究霍山石斛同一蒴果组培不同生长阶段遗传稳定性。方法：利用从20个随机引物筛选的3个稳定性较好的10碱基引物对已继代7-8次的H23种群进行RAPD检测。结果：发现不同阶段内遗传相似系数较大,在85.409 3%-98.360 6%,其中在原球茎、萌芽期和一叶期存在的变异比两叶期、开花期要显著,但程度都极其微弱。结论：来自同一蒴果的变异非常小,建立霍山石斛稳定的无性快繁系是切实可行的。Objective： To research the hereditary stability of Dendrobium huoshanense which were subcultured 7-8 times in the same tissue culture system. Method： Using three primers of arbitrary decamer oligonucleotide sequences from 20 primers for DNA amplification, random amplified polymorphic DNA （RAPD） were performed. Result： The genetic similarity coefficient was varied from 85.4% to 98.4%. The variation of protocorm, germination and monoleaf were rather more notable than that of bileaf and inflorescence. Conclusion： The variation of the same pod is quite small. It is feasible to set up the rapid propagation system of D. huoshanense.

关 键 词：随机扩增多态性DNA 霍山石斛 生长阶段 遗传稳定性 

分 类 号：S567.239[农业科学—中草药栽培]