塔斯品碱对小鼠S180肉瘤的抑制作用及其机制探讨  

Inhibitory effect of taspine on mouse S180 sarcoma and its mechanism

作　　者：张彦民[1] 贺浪冲[1] 王红英[1] 

机构地区：[1]西安交通大学医学院,陕西西安710061

出　　处：《中国中药杂志》2007年第10期953-956,共4页China Journal of Chinese Materia Medica

摘　　要：目的:研究塔斯品碱对小鼠S180肉瘤生长的抑制作用及其机制。方法:建立小鼠S180肉瘤动物模型,利用该模型观察塔斯品碱的体内抗肿瘤作用。通过免疫组织化学Elivision plus法检测肿瘤组织内微血管密度(MVD)及血管内皮细胞生长因子(VEGF),碱性成纤维细胞生长因子(bFGF),凋亡基因Bcl-2,Bax蛋白表达情况。结果:塔斯品碱对小鼠S180肉瘤具有抑制作用,瘤体血管内的VEGF,bFGF,Bcl-2,Bax蛋白表达逐渐下降,Bax/Bcl-2比值逐渐上升,存在良好的量效关系。结论:塔斯品碱能够抑制小鼠S180肉瘤的生长,其机制可能与降低瘤体组织内VEGF,bFGF,Bcl-2和Bax的蛋白表达,促进血管内皮细胞凋亡有关。Objective： To study the inhibition effect of taspine on mouse S180 sarcoma and its mechanism. Method： The mouse S180 sarcoma model was established and used to observe the antitumor activity of taspine. The mierovessel density and protein expressing of the VEGF, bFGF, Bcl-2 and Bax in the tumor were measured by immunohistochemistry. Result： Taspine showed antitumar activity on the mouse S180 sarcoma in a good dose-dependent manner. The inhibition rates on tumor of taspine at low, middle and high concentrations were 39.08%, 43.99% and 48.60% , respectively. The mierovessel density and protein expressing of the VEGF, bFGF, Bel-2 and Bax in the tumor were decreased compared with the negative control. The ratio of Bax to Bcl-2 was increased. Conclusion ： Taspine has anfitumor effect on the S180 sarcoma, and the mechanism may be through the way of decreasing the expressing of the VEGF, bFGF, Bcl-2 and Bax and inducing the vascular endothelial cell apoptosis.

关 键 词：塔斯品碱 S180肉瘤 血管生成 蛋白表达 

分 类 号：R285.5[医药卫生—中药学]