早期切痂对烧伤大鼠肺组织核因子-κB活化的影响  

Effect of escharectomy on rats'pulmonary NF-κB activation in early stage of burn injury

作　　者：李志清[1] 黄跃生[2] 杨宗城[2] 王甲汉[1] 

机构地区：[1]南方医科大学南方医院烧伤科,广州510515 [2]第三军医大学西南医院全军烧伤研究所/创伤,烧伤与复合伤国家重点实验室

出　　处：《中华急诊医学杂志》2007年第5期474-476,共3页Chinese Journal of Emergency Medicine

基　　金：国家重点基础研究发展规划基金(G1999054202)

摘　　要：目的探讨大鼠烧伤后早期切痂对肺组织核因子-κB（NF-κB）活化和促炎细胞因子表达的影响，进一步阐明烧伤早期切痂有利于控制全身炎症反应的机制。方法采用Wistar大鼠Ⅲ°35％TBSA烧伤模型，实验分正常对照组（A组）、烧伤后非切痂组（B组）和烧伤后早期切痂组（C组）。大鼠烧伤后12、24h凝胶电泳迁移率分析法（EMSA）检测肺组织NF-κB活性；逆转录．聚合酶链式反应（RT-PCR）检测肺组织TNF-α mRNA表达；酶联免疫吸附分析（ELISA）检测肺组织TNF-α含量。数据采用Excel统计分析软件行t检验。结果B组大鼠伤后12、24h肺组织NF-κB活性分别为（19．56±1．36）×10^4积分灰度值（A）和（15．23±1．94）×10^4A，均明显高于A组[（4．36±0．38）×10^4A，P〈0．01]。B组伤后12h肺组织TNF-α mRNA表达（1．37±0．47）较A组（0．28±0．04）升高（P〈0．01），TNF-α含量（1．88±0．75）ng／ml也明显高于A组[（0．62±0．05）ng／ml，P〈0．01]。C组肺组织NF-κB活性伤后12h为（7．12±1．15）×10^4A，24h为（6．33±0．90）×10^4A，分别较B组显著降低（P〈0．01）；C组大鼠肺组织伤后12、24h时TNF-α mRNA表达和含量均显著低于B组（P〈0．01）。结论严重烧伤可激活大鼠肺组织NF-κB，烧伤后早期切痂可有效抑制肺组织NF-κB活化，从而下调肺组织对致炎细胞因子的合成和释放，减轻机体全身炎症反应。Objective To investigate the effect of escharectomy on rats＇ pulmonary NF-κB activation and the expression of pulmonary proinflammatory cytokines in early stage of burn injury. Method Wistar rats were randomly divided into three groups： group A （control group）, group B （postburn without escharectomy）, group C （escharectomy at early stage of burn injury）. Thermal-injuried rats underwent 35% TBSA full-thickness burns. Activation of pulmonary NF-κB at 12 hours and 24 hours postburn was tested by electrophoretic mobility shift assay （EMSA）, and at the same time expressions of pulmonary TNF-α mRNA were measured by reverse transcription polymerase chain reaction （RT-PCR） and release of pulmonary TNF-α were assayed by enzyme-linked immunosorbent assay （ELISA）. Results Compared with control group, activity of pulmonary NF-κB in group B was markedly increased, reached （19.56±1.36）×10^4 A at 12 hours and （15.23±1.94）×10^4A at 24 hours, which was higher than that in group A [ （4.36±0.38）×10^4 A, P 〈 0.01]. Expressions of pulmonary TNF-α mRNA （1.37±0.47） and release of pulmonary TNF-α （1.88±0.75） ng/ml increased in group B significantly than those in group A [ （0.28±0.04）, （0.62±0.05 ） ng/ml] at 12 hours after thermal injury respectively （ P 〈 0.01 ） . But activity of pulmonary NF-κB in group C was decreased, which reached （7.12±1.15）×10^4 A at 12 hours after thermal injury and （6.33±0.90）×10^4 A at 24 hours after thermal injury, Expressions of pulmonary TNF-α mRNA and release of pulmonary TNF-α were lower in group C than those in group B （P 〈 0.01）. Conclusions Severe burn might activate pulmonary NF-κB , which uhimately lead to the release of cytokines from the lung. Escharectomy might inhibit pulmonary NF-κB activation and thereby down-regulate the inflammatory reaction in early stage of thermal injury.

关 键 词：烧伤 肺 核因子-ΚB 

分 类 号：R644[医药卫生—外科学]