土贝母中皂苷成分的分离及对K562细胞的作用  

Extraction of Tubeimosides from Bolbostemma paniculatum and Their Effects on Human Erythroleukamia K 562

作　　者：于超[1] 何俊琳[1] 邱宗荫[1] 

机构地区：[1]重庆医科大学感染性疾病分子生物学教育部重点实验室,重庆400016

出　　处：《西南大学学报（自然科学版）》2007年第4期89-95,共7页Journal of Southwest University(Natural Science Edition)

摘　　要：探讨土贝母皂苷类成分分离方法及对K562肿瘤细胞的体外诱导分化作用机制.采用LLC细胞毒检测为活性指导,通过系统分离,光谱分析确定结构,得到活性较高的土贝母皂苷Ⅰ、Ⅱ、Ⅲ和Ⅴ;选择结构上有差异的土贝母皂苷Ⅰ和Ⅲ为作用药物,观察其对K562细胞增殖及分化的影响.用流式细胞仪分析用药后细胞周期改变情况.结果表明,土贝母皂苷Ⅰ、Ⅲ与对照组比较,作用细胞5 d后,细胞增殖受到明显抑制,细胞形态出现中幼、晚幼粒细胞和幼红系的形态特征,对细胞内糖原、过氧化物酶和非特异性酯酶检测均为阳性,细胞周期多数被阻滞在S期以前,说明土贝母皂苷Ⅰ、Ⅲ可以促进肿瘤细胞分化,同时抑制其增殖.The isolation methods of saponin from Bolbosternrna paniculaturn （Maxim） Franquet and the anti-tumor mechanism of tubeimosides were investigated through inducing differentiation of human erythroleukamia K562. The cytotoxicity test on LLC cell line was used as bioactive guide. Systematic separation and spectrometry were performed to determine the molecular structures, and the tubeimosides Ⅰ,Ⅱ,Ⅲ and Ⅴ with high bioactivity were obtained. The tubeimosides I and III, which have different molecular structures, were supplemented to the medium in triplicate 2 days after K562 cells were plated. After incubation with the studied compounds for 5 days, the proliferation and differentiation of K562 cells were observed. Our results demonstrated that the studied compounds could inhibit the proliferation and promote the differentiation of K562 cells. The morphologic characteristics of myelocyte, metamyelocyte and normoblast were observed under a microscope. The hepatin, myeloperoxidase and α-naphthol acetate esterase were assayed as positive. The K562 cell was inhibited in G1 period by tubeimosides. It is clearly demonstrated that tube- imosides I and III could induce differentiation of tumour cells and inhibit their proliferation.

关 键 词：土贝母皂苷 K562 细胞分化 

分 类 号：S567.231[农业科学—中草药栽培]