毛竹“韧皮部结”发育过程中Ca^(2+)-ATP酶的超微定位(英文)  

Ultracytochemical Localization of Ca^(2+)-ATPase during the Phloem Ganglion Development in Phyllostachys edulis

作　　者：于芬[1] 丁雨龙[1] 尹增芳[2] 

机构地区：[1]南京林业大学竹类研究所,南京210037 [2]南京林业大学森林资源与环境学院,南京210037

出　　处：《西北植物学报》2007年第4期645-650,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家十五"科技攻关项目(2004BA506B01);国家自然科学基金项目(30271064)

摘　　要：用电镜和细胞化学技术对毛竹[Phyllostachys edulis(Carr.)H.De Lehaie]节部韧皮部结"发育过程中Ca2+-ATP酶进行了超微细胞化学定位研究.结果显示:在韧皮部结"形成期,仅细胞质膜和细胞核上具有很高的Ca2+-ATP酶活性;随着韧皮部结"的发育,发育期细胞质膜上的Ca2+-ATP酶活性较形成期有所降低,而细胞核上仍保持较高的Ca2+-ATP酶活性,胞间连丝、运输小泡膜上都具有Ca2+-ATP酶活性;发育后期,液泡膜及内质网上也开始出现Ca2+-ATP酶沉积物;成熟期的韧皮部结"细胞质膜上的Ca2+-ATP酶活性较发育期有所升高,并且在韧皮部结"成熟的过程中,细胞核、内质网、胞间连丝、质体膜和细胞质降解物上始终都有较高的Ca2+-ATP酶活性.实验结果表明韧皮部结"细胞具有活跃的生理代谢以及频繁的共质体运输和信息交流.The Ultracytochemical localization of Ca^2＋-ATPase during the phloem ganglion development in Phyllostachys edulis （Carr.） H. De Lehaie was studied with cytochemical technology. In the initiating phase of the phloem ganglion, the plasma membrane and nuclei have high Ca^2＋-ATPase activity. During the developmental phase,Ca^2＋-ATPase deposits in the plasma membrane decreased, but Ca^2＋-ATPase activity in the nuclei remained high. Ca^2＋-ATPase deposits were visualized in transfer vesicles and plasmodesmata. In the later period of the developmental phase, the distribution of Ca^2＋-ATPase appeared in the tonoplast and ER（endoplasmic reticulum）. During the maturation phase,Ca^2＋-ATPase activity in the plasma membrane is little higher than that of in developmental phase,and during the maturation of the phloem ganglion, the nuclei,plasmodesmata,ER, plastid membrane and degradation always have high Ca^2＋ -ATPase activity. These findings indicate that the phloem ganglion has active physiological metabolism, symplastic translocation and communication.

关 键 词：CA^2+-ATP酶 发育 韧皮部结 毛竹 生理功能 

分 类 号：Q944.62[生物学—植物学]