不同紫花苜蓿品种根瘤菌遗传多样性的PCR-SSCP分析  

Genetic Diversity on Rhizobia Isolated from Different Medicago sativa Varieties with PCR-SSCP Method

作　　者：丁小平[1] 韦革宏[2] 余建福[1] 曹鹏[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨陵712100 [2]陕西省农业分子生物学重点实验室,陕西杨陵712100

出　　处：《西北植物学报》2007年第4期678-683,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：全国优秀博士学位论文作者基金(200254);教育部新世纪优秀人才支持计划(NCET-04-0953);西北农林科技大学拔尖人才支持计划;国家自然科学基金(30470040)

摘　　要：用PCR-SSCP方法对分离自23个紫花苜蓿品种的42株供试根瘤菌和2株苜蓿根瘤菌参比菌株Sinorhizobium meliloti、Sinorhizobium medica进行遗传多样性分析.结果表明,供试的紫花苜蓿根瘤菌存在丰富的遗传多样性,在16S rDNA的V2～V3区段中有12种不同的等位基因,V4～V5区段有13种不同的等位基因,基因型27个;大部分供试菌株的基因型各不相同,来自同一品种菌株之间表现出不同的基因型,来自不同品种的菌株却表现出相同的基因型;9株供试菌株在V2～V3区段的基因型与参比菌株S.meliloti相同,所有供试菌株的基因型与参比菌株S.medica都不同.SSCP analysis of single strand PCR products of 16S rRNA gene V2 - V3 and V4 - V5 regions with 42 rhizobia isolated from 23 Medicago sativa varieties and 2 reference strains of S. meliloti and S. medica. The results suggested that there were 12 different alleles in V2-V3 region and 13 different alleles in V4-V5 region,27 genotypes in these two regions. Most of PCR-SSCP profiles were different each other. Besides, some strains from the same M. sativa variety showed different genotypes and same genotypes in different M. sativa varieties. In V2 -V3 region, 9 strains were uniform with S. meliloti and all tested strains were dissimilar with S. medica.

关 键 词：紫花苜蓿 根瘤菌 PCR—SSCP 遗传多样性 

分 类 号：Q939.114[生物学—微生物学] Q346.5