番茄叶片糖与转化酶的日变化研究  

Diurnal Variation of Invertase Activities and Sugar Content in Tomato Leaves

作　　者：吴正景[1] 程智慧[1] 孟焕文[1] 周艳丽[1] 徐强[1] 

机构地区：[1]西北农林科技大学园艺学院

出　　处：《西北植物学报》2007年第4期705-709,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金项目(30370977);国家十五"科技攻关项目"(2004BA516A09)

摘　　要：为探讨转化酶在叶片糖分含量和光合作用日变化中的作用,测定了番茄（Lycopersicon esculentum L.）叶片光合速率、转化酶活性、淀粉、蔗糖、葡萄糖和果糖含量,并分别分析了12：00前后它们间的相关关系.结果表明：番茄日间叶片净光合速率在10：00和16：00出现一大一小两个高峰;6：00～18：00叶片中淀粉和果糖呈现持续升高,而蔗糖和葡萄糖为先升后降趋势;光合速率同叶片蔗糖含量和胞质转化酶活性存在高度正相关,淀粉和果糖含量同光合速率未表现出显著相关性.由此可知,胞质转化酶在蔗糖代谢方面有明显的作用;果糖可能是通过抑制胞壁转化酶活性,促进了蔗糖外运.The diurnal variations of photosynthetic rate, invertase activities and sugar content in tomato leaves were measured from 6：00 to 18：00. The results showed：there were two net photosynthetic rate high points at 10：00 and 16：00, and starch and fructose content kept increasing, however, sucrose and glucose content decreased after 12：00. There were high correlation between photosynthetic rate,sugar content and cytosolic invertase activity;Photosynthetic rate wasn＇t significant negative correlated with starch and fructose content in leaves. Cytosolic invertase would play a key role in avoiding superfluous sucrose accumulation in cytoplasm to inhibit photosynthetic rate. Fructose of high concentration might restrain the activity of cell wall invertase,and facilitate sucrose transiting out.

关 键 词：番茄叶片 光合速率 转化酶 糖分 

分 类 号：Q945.11[生物学—植物学] S641.2[农业科学—蔬菜学]