紫茎泽兰花蕾发育过程中类Beclin1基因的克隆表达分析(英文)  

Cloning and Characterization of Beclin1-like Gene in Eupatorium adenophorum Buds

作　　者：王敏 刘英[1] 李思滨[1] 姜洋[1] 祁长青[1] 王俐[1] 祖元刚[1] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040

出　　处：《植物研究》2007年第4期460-464,共5页Bulletin of Botanical Research

摘　　要：以紫茎泽兰不同发育时期花蕾为实验材料,通过光学显微观察、DNA ladder检测表明紫茎泽兰花蕾发育过程中绒毡层有PCD现象发生,自紫茎泽兰花蕾中克隆长为679bp的Beclin1 cDNA基因部分片段,同烟草叶片的Beclin1基因序列(AY701316)同源性为98%,通过Northern blotting分析,在紫茎泽兰花蕾发育过程中细胞程序性死亡(PCD)相关Beclin1基因表达在花蕾发育第Ⅱ期和Ⅲ期较为旺盛,且在花蕾发育第Ⅱ期最强烈。本研究首次克隆并证实紫茎泽兰花蕾Beclin1基因与PCD有一定的相关性,为分析紫茎泽兰入侵机理提供新的思路和手段。It was found in the present study that programmed cell death （PCD） took place during the tapetum degenerating and pollen developing by microscopical observation and DNA ladder detection in Eupatorium adenophorum buds. Simultaneously, Beclin1 -like gene was cloned from E. adenophorum buds at four different growth stages by RT-PCR method. The partial Beclin1 -like gene （679 bp） was 98% similar to that of tobacco leaves （AY701316）. Northern blotting showed that the expression of Beclinl -like gene in the stage Ⅱ was the strongest. The Beclin1 -like gene was cloned in E. adenophorum buds for the first time and some relationships between the Beclin1 -like Gene and PCD during the procreation of E. adenophorum were demonstrated. In addition, the results provided a new idea to further explore the invading mechanism of E. adenophorum.

关 键 词：紫茎泽兰 花蕾 细胞程序性死亡(PCD) Beclin1基因 NORTHERN blotting分析 

分 类 号：Q51[生物学—生物化学]