清脑宣窍滴丸对大鼠局灶脑缺血再灌注炎症因子含量的影响  

Effect of Clearing Brain and Releasing Orifices Dripping Pills on Inflammatory Factors Contents in Focal Cerebral Ischemia-reperfusion Injury Rats

作　　者：柳洪胜[1] 张允岭[1] 刘雪梅[2] 娄金丽[1] 郑宏[1] 黄启福[2] 

机构地区：[1]北京中医药大学东方医院,北京100078 [2]北京中医药大学基础医学院,北京100029

出　　处：《中华中医药杂志》2007年第5期328-330,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：教育部新世纪优秀人才支持计划(No.NCET-05-0139)

摘　　要：目的:探讨清脑宣窍滴丸对大鼠局灶脑缺血再灌注组织肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、细胞间黏附分子-1(ICAM-1)含量的影响。方法:21只大鼠随机均分假手术组、缺血再灌注组、清脑宣窍滴丸组,采用线栓法制备大鼠左侧大脑中动脉缺血再灌注模型,缺血1.5h,再灌注12h,Zea longa法评定神经功能缺损状况,光镜观察脑片病理形态改变,ELISA方法测定脑组织TNF-αI、L-1βI、CAM-1的含量。结果:脑缺血再灌注12h模型组病变侧大脑皮层结构层次破坏严重,神经细胞肿胀,核碎裂、溶解、固缩或消失,神经功能明显缺损,模型组中TNF-α、IL-1β、ICAM-1含量分别为(5.77±0.30),(0.91±0.14),(5.45±0.60),其异常升高与假手术组比较有显著意义(P<0.01或P<0.05),清脑宣窍滴丸组中TNF-α、IL-1β、I-CAM-1含量分别为(3.59±0.24),(0.70±0.20),(3.75±0.46),与模型组比较明显下降(P<0.01或P<0.05)。结论:脑缺血再灌注TNF-αI、L-1βI、CAM-1的异常升高与组织损伤密切相关;清脑宣窍滴丸对其异常升高具有下调作用,从而有效的抑制了炎症反应,减轻脑缺血再灌注损伤。Objective： To investigate the effect of Clearing Brain and Releasing Orifices Dripping Pills on TNF-α、IL-1β and ICAM-1 contents in focal cerebral ischemia-reperfusion injury rats. Methods： Thirty-six rats are divided at random into three groups： sham operated group, model group （ischemia reperfusion group） and treated group. Suture-occluded method was used to block the left middle cerebral artery, make ischemia for 1.5 h, after reperfusion for 12 h, the Zea longa method was used to evaluate the neurologic impairment and ELISA method to detect the content of TNF-α、IL-1β、ICAM-1 in brain tissue , observe the pathologic change in tissue at the same time. Result： Disorganization of structure layer of cerebral cortex is severity after reperfusion for 12 h, pathological change in tissue showed that nerve cell swelling, and there was fragmentation, dissolve, pyknosis or disappear of cell nucleus. Neurologic impairment is obvious. The content of TNF-α、IL-1β、ICAM-1 in model group individually is （5.77 ± 0.30）, （0.91 ± 0.14）, （5.45 ± 0.60）, increased when compared with sham operated group （ P 〈 0.01 or P 〈 0.05）, while the content of TNF-α、IL-1β、ICAM-1 in treated group individually is （5.77 ± 0.30）, （0.91 ± 0.14）, （5.45 ± 0.60）, decreased when compared with model group （ P 〈 0.01 or P 〈 0.05）. Conclusion： the tissue damage after cerebrla ischemia-reperfusion is closely related to the increase of TNF-α、IL-1β、ICAM-1, while clearing brain an releasing orifices dripping pills can decrease the content of inflammatory factors in tissue and inhibit the inflammatory reaction and lessen the brain ischemia-reperfusion injury.

关 键 词：脑缺血再灌注损伤 肿瘤坏死因子-Α 白细胞介素-1Β 细胞间黏附分子-1 清脑宣窍滴丸 

分 类 号：R285.5[医药卫生—中药学]