他莫昔芬对单侧输尿管梗阻大鼠肾间质纤维化的保护作用  

Effect of Tamoxifen on interstitial fibrosis in UUO rats

作　　者：姚刚[1] 许宏岳[1] 杨春[1] 吴限[1] 徐玲玲[1] 

机构地区：[1]南京医科大学第二附属医院肾脏科,江苏南京210011

出　　处：《南京医科大学学报（自然科学版）》2007年第5期480-482,I0002,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省教育厅资助项目(03KJD320125)

摘　　要：目的:探讨他莫昔芬对肾组织纤维化的影响及作用机制。方法:雌性SD大鼠,建立单侧输尿管梗阻模型(UUO),设:①模型组(n=6),行左侧输尿管结扎术,0.3ml无菌生理盐水灌胃,每天2次;②治疗组(n=6),他莫昔芬5mg/kg(0.3ml无菌生理盐水配置)灌胃,每天2次;③假手术组(n=6)。术后第9天处死各组大鼠。采用光镜和免疫组化观察肾组织纤维化和α平滑肌肌动蛋白(α-SMA)表达的变化。结果:①治疗组大鼠肾间质纤维化程度较模型组明显减轻。②UUO模型第9天时大鼠肾组织α-SMA表达明显增强,采用他莫昔芬治疗后,肾组织α-SMA表达的异常增强能得到有效抑制。结论:昔芬类药物具有减轻肾组织纤维化的作用,而这一作用与其能有效阻抑肾组织上皮细胞-间充质细胞转分化(EMT)有关。Objective：To investigate the effects of Tamoxifen on renal interstitial fibrosis in UUO rats. Methods：Feamale SD rats were divided into 3 groups at random：（1）UUO group（6 rats）, （2）the treated group（6 rats）：UUO ＋ Tamoxifen 10 kg/（mg·d） day by gastrogavage, （3）sham operated group （6 rats）. Kidney were harvested at day 9 after UUO. The renal tissues were examined by HE, PAS and Masson. Tissue α-SMA protein expression was determined by Immunohistochemistry. Results：UUO for 9 days significantly activated α-SMA protein expression, induced interstitial fibrosis and tubulointerstitial lesion in obstructed kidney of UUO group, compared with sham operated group rats. Conversely daily administration of Tamoxifen significantly inhibited α-SMA expression and decreased interstitial fibrosis and tubulointerstitial lesion in obstructed kidney of treated group. Conclusion：Tamoxifen had beneficial effects on reduction of renal interstitial fibrosis in SD rats with UUO, and this effect may be associated with Tamoxifen inhibits α-SMA expression.

关 键 词：肾脏间质纤维化 输尿管梗阻 他莫昔芬 

分 类 号：R692.9[医药卫生—泌尿科学]