实时荧光定量PCR检测不同舌苔黏膜组织中PLOD1基因表达水平  

Test of PLOD1 gene expressions in different types of mucous membrane of tongue coating by PCR

作　　者：吴慧平[1] 范媛[2] 丁兴[1] 许冬青[1] 詹瑧[1] 

机构地区：[1]南京中医药大学基础医学院,江苏210029 [2]江苏省口腔医院

出　　处：《北京中医药大学学报》2007年第4期249-251,255,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.30472123)

摘　　要：目的应用实时荧光定量PCR检测不同舌苔黏膜组织中赖氨酰羟化酶(PLOD1)基因表达,分析该基因表达量与不同舌苔之间的关系。方法通过RT-PCR逆转录得到正常胎儿和五种病理性舌苔黏膜组织的cDNA,再经实时荧光定量PCR,测定PLOD1基因在不同舌苔黏膜组织的定量表达,观察其表达量的变化趋势。结果检测发现PLOD1基因在病理性舌黏粘膜组织中表达量递减趋势为:白薄苔组>黄薄苔组>黄厚苔组>白厚苔组>无苔组>正常组;其中再与无苔组PLOD1基因拷贝比值倍比分别呈2.97、2.68、2.21、1.50倍。结论PLOD1基因在病理性舌苔黏膜组织表达量的改变,影响舌苔性质。Objective To detect the gene expressions of procollagen-lysine 1, 2-oxoglutarate 5- dioxygenase 1 （ PLOD1 ） in different types of mucous membrane of tongue coating by using real time fluorescent quantitative PCR and analyze the relationship between the expression levels and tongue coating. Methods The chief cDNA were collected from natural fetus and five types of pathologic mucous membrane of tongue coating by RT-PCR. The gene expression levels and tends in different types of mucous membrane of tongue coating were detected and observed by the real time fluorescent quantitative PCR. Result The result of gene expression levels of PLOD1 in pathologic mucous membrane of tongue coating showed a descending tend as follows. White thin coating group 〉 yellow-thin coating group 〉 yellow-thick coating group 〉 white thick coating group 〉 no coating group 〉 normal coating group. The multiple proportion of copy ratio of PLOD1 showed by 2.97, 2.68, 2.21 and 1.50 times respectively compared with the no coating group. Conclusion The changes of expression levels of PLOD1 in pathologic mucous membrane of tongue coating can influence the quality of tongue coating.

关 键 词：舌苔 实时荧光定量PCR 逆转录聚合酶链反应 赖氨酰羟化酶基因 

分 类 号：R285.5[医药卫生—中药学]