不同程度炎症的人牙龈树突状细胞的数量研究  

Quantitative evaluation of dendritic cells in healthy and diseased human gingiva

作　　者：刘宇[1] 林松杉[1] 章禾[1] 刘奕[1] 

机构地区：[1]海军总医院口腔科,北京100037

出　　处：《临床口腔医学杂志》2007年第5期283-285,共3页Journal of Clinical Stomatology

基　　金：首都医学发展科研基金资助(2003-3033);海军总医院科研基金资助(2002-014)

摘　　要：目的:比较不同程度炎症的人牙龈树突状细胞的数量。方法:使用CD1a单克隆抗体和DC-SIGN单克隆抗体,分别对人牙龈郎格罕细胞(Langerhans cells,LCs)和真皮树突细胞(dermal dendritic cells,DDCs)作免疫组织化学染色,对正常(11例)、牙龈炎(14例)、牙周炎(15例)三组样本的两种细胞进行计数并比较。结果:LCs主要分布在牙龈上皮的基底层及棘层,高倍视野下三组样本的细胞数量分别是13±3.3、21±5.1、42±4.8(p<0.01);DDCs主要分布在牙龈上皮的基底膜以下,高倍视野下三组样本的细胞数量分别是27±6.3、33±5.1、76±4.8(p<0.01)。结论:牙龈的LCs和DDCs的数量与牙周炎症发展过程有关。To evaluate the quantity of Langerhans cells （LCs）and dermal dendritic cells （DDCs）in hea- lthy and diseased human gingiva.Method： LCs and DDCs in gingiva were immunohistochemical stained with monoclonal CDla antibody and monoclonal DC-SIGN antibody respectively. The number of positively stained cells were counted in six random high power fields （10×40）and statistically analyzed.Result： LCs located in the epithelial stratum basale and stratum spinosum. The CD1a ＋ LCs were 13±3.3/HPF,27±5.1/HPF,42±4.8/HPF in healthy group, gingivitis group and periodontitis group （p〈0.01）.DDCs mainly located in the subepithelial gingival connective tissue. The DC-SIGN＋DDCs were 27±6.3/HPF, 33±7.7/HPF, 76±11.4/HPF in three groups （p〈0.01）.Conclusion：The number of LCs and DDCs increased in diseased sites of oral epithelium.

关 键 词：郎格罕细胞 牙龈炎 牙周病 

分 类 号：Q813.1[生物学—生物工程]