葡萄糖酸镁对离体大鼠心肌缺血再灌注细胞凋亡的抑制效应  

Inhibitory effects of magnesium gluconate on the apoptosis of myocardial cells in rats with ischemia reperfusion injury in vitro

作　　者：贺欣[1] 陶贵周[1] 齐志敏[2] 

机构地区：[1]辽宁医学院附属第一医院心内科,辽宁省锦州市121001 [2]辽宁医学院药理教研室,辽宁省锦州市121001

出　　处：《中国组织工程研究与临床康复》2007年第8期1465-1468,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：目的:观察葡萄糖酸镁对离体大鼠心肌缺血再灌注损伤心肌细胞凋亡的影响。方法:实验于2005-10/2006-01在辽宁医学院药理实验室完成。选用雄性SD大鼠48只,体质量250～300g,按随机排列表法将大鼠分为对照组、缺血再灌注组、葡萄糖酸镁组,每组16只,建立Langendorff离体大鼠心肌缺血再灌注损伤模型。(1)每组各取8只:①对照组:改良的K-H缓冲液持续灌流110min。K-H缓冲液成分(mmol/L)如下:NaCl118.1,NaHCO325.0,KH2PO41.2,MgSO40.6,CaCl22.0,KCl4.7,Glucose11.0。②缺血再灌注组:改良的K-H缓冲液持续灌注至各项指标稳定后,约20min,停灌30min,再灌60min。③葡萄糖酸镁组:灌注方法同缺血再灌注组,但将K-H缓冲液内加葡萄糖酸镁2.4mmol/L。取左室心肌标本测总超氧化物歧化酶活性,丙二醛、Ca2+含量。(2)每组其余8只:①对照组K-H缓冲液持续灌流170min。②缺血再灌注组和葡萄糖酸镁组持续灌注至各项指标稳定后停灌30min,再灌注120min。观察对细胞凋亡的影响。(3)组间计量资料差异比较采用单因素方差分析。结果:SD大鼠共48只均进入结果分析。①缺血再灌注组大鼠心肌组织中丙二醛、Ca2+含量较对照组明显升高(P<0.01),总超氧化物歧化酶活性较对照组明显降低(P<0.01)。②葡萄糖酸镁组与缺血再灌注组比较,总超氧化物歧化酶活性明显升高(P<0.01),丙二醛、Ca2+含量明显降低(P<0.01)。③缺血再灌注组细胞凋亡指数显著高于对照组(P<0.01)。④葡萄糖酸镁组细胞凋亡指数与缺血再灌注组比较显著降低(P<0.01)。结论:葡萄糖酸镁有抑制心肌缺血再灌注损伤中心肌细胞凋亡的作用。其机制可能与葡萄糖酸镁减轻钙超载、清除氧自由基、减少脂质过氧化有关。AIM： To study the effect and mechanism of magnesium gluconate on cardiac myocyte apoptosis in myocardial ischemia reperfusion injury （MIRI） with isolated rat heads. METHODS： The experiment was conducted in the Department of Pathology, Jinzhou Medical University from October 2005 to January 2006. A total of 48 male SD rats weighted 250-300 g were selected to isolate the heads and establish myocardial MIRI models by linking to Langendorff perfusion apparatus, and then randomly divided into 3 equal groups： control group, I/R group and magnesium gluconate group, with 16 rats in each.（1）Eight rats in each group were subjected to continuous perfusions： ①Control group： modified K-H buffer （components： NaCl 118.1, NaHCO3 25.0, KH2PO4 1.2, MgSO4 0.6, CaCl2 2.0, KCl 4.7, Glucose 11.0, unit： mmol/L） for 110 minutes.②I/R group： The rats were treated with continuous perfusion of modified K-H buffer until 20 minutes later, all the index were stable, and then the reperfusion was given for 60 minutes following being stopped for 30 minutes.③ Magnesium gluconate group： The perfusion method was the same as I/R group, and K-H buffer was added with 1.8 mmol/L magnesium gluconate. Samples of left ventricle myocardium were taken out to detect the activity of total superoxide dismutase （SOD） and the contents of Ca^2＋ and malondialdehyde （MDA）.（2）The other 8 rats in each group were selected.①Control group was given continuous perfusion for 170 minutes. ②I/R group and magnesium gluconate group were subjected to 120 minutes reperfusion after 30 minutes of stopping perfusion when the markers were stable by continuous perfusion. Myocardium apoptosis ware investigated in each group. （3）The measurement data were compared with single factor analysis of variance. RESULTS： All the 48 SD rats were involved in the analysis of results. ①Compared with control group, the contents of MDA and Ca^2＋ were significantly increased （P 〈 0.01） while the activity of total SOD was signifi

关 键 词：心肌缺血 再灌注损伤 细胞凋亡 

分 类 号：R542.2[医药卫生—心血管疾病]