马齿苋水提液对D-半乳糖致衰老模型小鼠心肌线粒体的保护作用(英文)  

Protective effect of Portulaca water extract on the myocardial mitochondria of aging model mice induced by D-galactose

作　　者：欧芹[1] 魏晓东[1] 王丹[1] 张鹏霞[1] 

机构地区：[1]佳木斯大学基础医学院生化教研室,黑龙江省佳木斯市154007

出　　处：《中国组织工程研究与临床康复》2007年第8期1570-1572,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:线粒体是细胞内主要耗氧部位,是产生自由基的主要场所,也是内源性自由基攻击的靶部位,其结构和功能会随着年龄的增加而改变,逐步导致全身性生理功能下降。马齿苋(Portulaca oleraceaL.)有长寿菜之称,营养丰富,又有抗自由基作用,其抗衰老作用是否与心肌线粒体的保护有关值得探讨。目的:观察马齿苋水提液对衰老模型小鼠心肌线粒体磷脂的脂质过氧化和呼吸链酶活性的影响,分析其心肌保护作用途径。设计:完全随机分组设计,对照动物实验。单位:佳木斯大学基础医学院生化教研室。材料:①于2004-01/08在佳木斯大学实验动物中心完成动物饲养和实验,在生物化学教研室完成动物的处死、心脏取材、线粒体的制备,在生物化学教研室、大学实验中心、化学与药学院完成指标的测定。选用成年纯系昆明种健康小白鼠30只,雌雄不拘。②按随机摸球法分为3组:青年对照组8只,衰老模型组和模型给药组各11只。③马齿苋由佳木斯市中医药研究所提供,本校生药教研室鉴定,制成含生药浓度1kg/L水提液。心磷脂标准品由美国Sigma公司提供;丙二醛、Ca2+-ATP酶活性测定试剂盒由南京建成生物工程研究所提供。方法:①衰老模型组每日颈背部皮下注射D-半乳糖100mg/kg;马齿苋多糖组除注射D-半乳糖外,同时按含生药13g/(kg·d)灌服马齿苋水提液;青年对照组每日皮下注射等容量的生理盐水。连续给药30d,于最后一次给药次日处死,迅速取心脏,备用。②参考南京建成生物工程研究所提供的方法制备线粒体。按试剂盒说明测定线粒体内丙二醛含量及Ca2+-ATP酶活性;采用高效液相色谱法测定线粒体膜磷脂中心磷脂相对含量;采用伍期专的方法测定复合体Ⅰ及复合体Ⅱ+Ⅲ活性。③计量资料差异比较采用方差分析和t检验。主要观察指标:各组小鼠心肌线粒体膜磷脂组成、线粒体丙二�BACKGROUND ： Mitochondria are not only the important place for consuming oxygen and producing free radical, but also an aggressive target place by endogenous free radical. The changes of structure and function of mitochondria will be take place with aging. Portulaca （Portulaca oleracea L.） is usually called as the macrobiotic vegetable. Portulaca is eutrophic and anti-free radical. It is worth exploring whether the anti-aging action of Portulaca is correlated with its protection on myocardial mitochondria. OBJECTIVE： To observe the effects of the Portulaca water extract on the lipid peroxidation myocardial mitochondrial phospholipid and the activity of respiratory chain enzymes in aging model mice, and analyze the pathway of protective effect on myocardial mitochondria. DESIGN： A completely randomized design and controlled animal experiments SETTING： Department of Biochemistry, School of Basic Medical Sciences, Jiamusi University MATERIALS ： ①The animals were raised and the experiments were completed in the Experimental Animal Center of Jiamusi University from March 2003 to August 2004. The animals were killed, hearts were removed and mitochondria were harvested in the Department of Biochemistry; The indexes were determined in the Department of Biochemistry, experimental center and the College of Chemistry and Pharmacology. ② Thirty healthy adult Kunming mice （either male or female） were divided into 3 groups by random feeling ball method： young control group （n =8）, aging model group （n =11） and Portulaca treated group （n =11 ）.③ Portulaca was offered by Jiamusi Institute of Chinese Herbs, and appraisement by the Department of Crude Drug of Jiamusi University. Portulacas were made into water extract （crud drug 1 kg/L）. Standard cardiolipin was offered by Sigma Company （USA）, kits for malonaldehyde （MDA） and the activity of Ca^2＋-adenosine triphosphatase （Ca^2＋-ATPase） were provided by Nanjing Jiancheng Bioengineering Institute. METHODS： ①The

关 键 词：线粒体 心脏/药物作用 马齿苋 衰老/药物作用 

分 类 号：R339.3[医药卫生—人体生理学]