核转录活性测定法研究黄芪当归及单组分对肾病鼠白蛋白基因的调控机制  

Chinese herbs improve transcription of the gene for albumin in nephrotic syndrome rats

作　　者：李丽英[1] 陈孟华[1] 王海燕[1] 童坦君[2] 刘星谐 

机构地区：[1]北京医科大学第一医院肾内科,100034 [2]北京医科大学第一医院基础医学院生物化学与分子生物学系,100034 [3]上海医科大学药学院

出　　处：《中华肾脏病杂志》1997年第1期25-28,共4页Chinese Journal of Nephrology

基　　金：卫生部科学基金

摘　　要：目的 探讨黄芪当归的治疗机理,并试图寻找黄芪的有效作用组分。方法 采用核转录活性测定(nuclear run-on assay)和分子杂交的方法观察了肾病综合征大鼠肝脏白蛋白基因的转录活性和白蛋白mRNA的表达水平。结果 肾病组转录活性高于对照组,而黄芪当归治疗组又高于肾病组。白蛋白mRNA的表达水平的变化趋势与之相吻合。用单味黄芪或黄芪的三个单组分治疗的各组大鼠白蛋白基因转录活性及mRNA表达水平与肾病组比无显著差异,但在血浆白蛋白水平有显著差异,且黄芪多糖Ⅱ的作用效果最强。结论 黄芪当归合剂能促进白蛋白基因转录活性,而单味黄芪和黄芪的三个组分改善肾病鼠蛋白质代谢紊乱不通过该机制。Objective To investigate the role of Astragali and Angelica (A&A) on improving the level of albumin and to find the eflective components of Astragali. Methods Male S. D. rats were divided into 7 groups: 1. normal control (C); 2. nephrotic control (N>; 3. therapy with A&A (A&A); 4. Astragali alone (A); 5. Astragali polysac-charide Ⅰ (APⅠ); 6. AP Ⅱ ; 7. Astragali glucoside (AG), Results The level of serum albumin,albumin mRNA and albumin gene transcription were measured by biochemistry, Northern blot hybridization, nuclear run-on assay and quantity in laser density screening. The level of serum albumin in N was significantly lower than C. The serum albumin concentration in each therapy group was higher than N group. The transcription of the albumin gene was higher in N than in C and highest in A&A. The alterations of northern blot hybridization were same as the transcription results. But both the level of albumin mRNA and the transcription of albumin gene in A, AP I , AP I and AG were no change compared with N. Conclusion A&A increases albumin mRNA expression at least in part by improving the rate of gene transcription, which participate the protection of the decrease of serum albumin in NS. But the effect of A, AP 1 , AP I and AG may mediated by other unknown mechanisms.

关 键 词：肾病综合征 白蛋白 基因 转录调控 中草药 

分 类 号：R277.520.5[医药卫生—中医学] R285.5