高血压患者血小板聚集功能与纤维蛋白原基因多态性的相关性研究  

The relationship between platelet aggregation function and Bβ-fibrinogen gene-455G/A polymorphism in hypertensive patients

作　　者：卞秋武[1] 林海龙[2] 张静[2] 杨红梅[1] 王晓亮[1] 王庆胜[1] 柳东田[1] 徐鹤[2] 唐学杰[3] 

机构地区：[1]河北医科大学附属秦皇岛第一医院心内科,河北秦皇岛066000 [2]大连大学附属大连中心医院心内科,辽宁大连116033 [3]大连医科大学附属二院,辽宁大连116033

出　　处：《中华老年心脑血管病杂志》2007年第4期232-234,共3页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

摘　　要：目的观察高血压患者血小板聚集功能变化是否与Bβ纤维蛋白原(Fib)基因启动子区-455G/A多态性有关。方法选取108例未经治疗的高血压患者,采用全血电阻抗法测定二磷酸腺苷(ADP)及花生四烯酸(AA)诱导的血小板聚集功能,并用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)方法测定BβFib基因启动子区-455G/A多态性,将其分为GG组和GA+AA组,比较两组血小板聚集功能变化。结果GA+AA组Fib浓度高于GG组(P<0.05),ADP诱导的血小板聚集能力GA+AA组低于GG组(P<0.05);而AA诱导的血小板聚集能力呈现GA+AA组高于GG组的趋势(P>0.05)。结论ADP诱导的血小板聚集功能与BβFib基因启动子区-455G/A多态性有关,GG基因型患者血小板聚集功能强于AA及GA基因型患者。但AA诱导的血小板聚集功能与其的关系呈现相反的趋势。Objective To investigate the relationship between platelet aggregation function and Bβ-fibrinogen gene -455G/A polymorphism in hypertensive patients. Methods Platelet aggregation function induced by ADP and arachidonic acid（AA） was determined by whole blood impedance method in 108 hypertensive patients. The -455G/A polymorphism of Bβ-fibrinogen was screened by PCR-RFLP. Platelet aggregation function was analyzed for groups genotype GG and genotype （GA ＋ AA）. Results Fibrinogen concentration in group GA ＋ AA was higher than that in group GG（ P 〈 0.05） .Platelet impedance（ohm） induced by ADP in group GG was higher than that in group GA ＋ AA （ P 〈 0.05）. Platelet impedance induced by AA in group GA ＋ AA was higher than that in group GG（ P 〉 0.05） .but there was no statistical difference. Conclusion Platelet impedance（ohm） induced by ADP correlates with the -455G/A polymorphism on the promoter region of Bβ- fibrinogen gene. Platelet aggregation function in hypertensive patients with genotype GG is stronger than that in patients with A455 allele, while there is a contrary tendency for platelet aggregation function induced by AA.

关 键 词：高血压 血小板聚集 纤维蛋白原 聚合酶链反应 多态性 限制性片段长度 

分 类 号：R544.1[医药卫生—心血管疾病]