土壤拮抗微生物对几种草莓病原菌的拮抗作用测试  

Bioassay of antagonistic microorganisms from soil against several strawberry disease-causing agents

作　　者：赵秀娟[1] 徐文桥[1] 张凤巧[1] 高坡[1] 蒋继志[1] 杜洪忠[1] 王树桐[1] 

机构地区：[1]河北农业大学植物保护学院河北省病虫害生物防治工程技术研究中心,河北保定071001

出　　处：《中国农业科技导报》2007年第2期77-81,共5页Journal of Agricultural Science and Technology

基　　金：河北省自然科学基金项目(C200500230);河北省教委博士科研启动基金项目(971102);河北农业大学大学生科技创新基金项目(07-ZR-076)

摘　　要：测试了分离自60份土样中植物根际微生物对草莓灰霉病菌和草莓重茬病菌的拮抗作用,获得了71个对2种靶标病原菌均表现拮抗作用的菌株。其中44个菌株对Botrytis cinerea Pers有拮抗作用;33个菌株对Pestaloti-opsis photiniae有拮抗作用;7个菌株对2种植物病原真菌均有明显的拮抗作用(抑菌带≥5 mm)。用上述7个拮抗菌株及其发酵液对B.cinerea Pers,P.photiniae,R.solona,Fusarium oxysporu和Verticillium dahliae进一步抑菌测定,结果表明:7个菌株及其发酵液对5种病原真菌均有较强的拮抗作用,具有较广谱的抑菌效果。用拮抗微生物处理后的病原菌厚垣孢子比空白对照病原菌的产生量大,菌丝肿胀变形、细胞质凝聚。Seventy microorganism isolates from 60 soil samples were evaluated for the antagonistic activity against Botrytis cinerea Pers, Pestalotiopsis photiniae. Results showed that 44 isolates showed antagonistic activity against B. cinerea Pers and 33 isolates against P. photiniae. The pathogen inhibition effed on both B. cinerea Pers and P. photiniae was achieved by 7 of antagonistic microorganisms in 70 isolates （inhibition zone ≥5 mm on average）. These 7 isolates were tested for the inhibition effect against B. cinerea Pers, P. photiniae, Rhizodonia solani, Fusarium oxysporum and Verticillium dahliae. The results showed that all the 7 isolates had broad-spedrum antifungal adivity on these 5 pathogens. These 5 pathogens developed more chlamydospores, the mycelia were bulging vacuolization and their cytoplasm congregated when the pathogens were treated with the antagonistic microorganisms.

关 键 词：拮抗微生物 草莓灰霉病 草莓重茬病 

分 类 号：S432.44[农业科学—植物病理学] S432.95[农业科学—农业昆虫与害虫防治]