次声暴露对豚鼠听力影响的实验研究  

Effects of infrasound on the auditory function of guinea pigs

作　　者：金娟[1] 刘静[1] 牟翔[1] 陈景藻[1] 

机构地区：[1]第四军医大学西京医院理疗科,西安710032

出　　处：《中华物理医学与康复杂志》2007年第5期310-313,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：军队指令性课题(01L071)

摘　　要：目的探讨以听觉脑干反应(ABR)和畸变产物耳声发射(DPOAE)为检测指标检测次声作用后对豚鼠听觉的影响。方法暴露条件为16 Hz、90 dB及16 Hz、130 dB。在次声舱里暴露2 h/次,1次/ 1 d,分别于1,7,14,21,28 d后分组检测豚鼠ABR和DPOAE。结果次声暴露后,豚鼠ABR反应阈有不同程度的升高。16 Hz、90 dB次声暴露1,28 d组ABR听觉反应阈与对照组相比差异有统计学意义(P<0.05)。16 Hz、1 30 dB次声暴露14,21,28 d组动物ABR听觉反应阈与对照组相比差异有统计学意义(P<0.01)。16 Hz、90 dB次声暴露后,各时间点豚鼠DPOAE的幅值在各频率点差异无统计学意义(P>0.05)。16 Hz、130 dB作用21 d组和28 d组DPOAE的幅值在各频率点差异有统计学意义(P<0.01)。结论进一步揭示了次声对豚鼠听觉的作用机制和量效关系,为全面研究次声对听觉系统的作用效果和防护标准问题奠定了一定基础。Objective To observe the effects of exposure to infrasound on auditory function of guinea pigs as reflected by the brain stem response （ABR） and distortion product otoacoustic emission（ DPOAE）. Methods Seventy-two Guinea pigs were used in this study, of them, 12 served as controls and 60 were divided into 2 experimental subgroups. ABR and DPOAE were detected after exposure to infrasound stimulation at 16 Hz, 90 dB or 16Hz, 130dBfor2hoursadayforl,7, 14,21 and28days. Results The threshold of ABR after exposed to infrasound at 16 Hz,90 dB in 1 day and 28 days was higher than the controls （P 〈 0.05 ）. The same change was observed at 16 Hz, 130 dB on 14,21 and 28 days（ P 〈 O. O1 ）. The DPOAE was not different between the experimental and the control groups after exposed to infrasound with 16 Hz,90 dB （P 〉 0.05 ） , but significant difference was observed with 16 Hz 130 dB infrasound exposure on the 21st and, 28th days when compared with the controls （ P 〈0.05）. Conclusion Exposure to infrasound has significant impact on auditory function of the guinea pigs, which is dose-dependent.

关 键 词：次声 听觉系统 听觉脑干反应 畸变产物耳声发射 豚鼠 

分 类 号：R764[医药卫生—耳鼻咽喉科]