高+Gx环境对猴颞肌c-jun蛋白表达影响的研究  

Changes of c-jun expression in temporalis muscles caused by hypergravity in Rhesus macaque

作　　者：汤楚华[1] 施生根[1] 张建中[2] 牛忠英[1] 李冬[2] 

机构地区：[1]北京解放军306医院全军口腔疾病诊治中心 [2]北京解放军306医院病理实验科

出　　处：《实用口腔医学杂志》2007年第3期353-355,共3页Journal of Practical Stomatology

基　　金：全军"十一五"计划课题项目基金资助(编号:06MA004);国家自然科学基金资助(编号:30672319)

摘　　要：目的:观察高过载(+Gx)环境对颞肌组织病理学及c-jun蛋白表达的影响。方法:以9只雄性猕猴为对象,按受试Gx环境及持续时间随机分4组,对照组为+1Gx、300s(2只);实验组为3组,分别为+15Gx、200s(2只);+18Gx、165s(2只);+21Gx、140s(3只)。采用病理学和免疫组织化学方法,观察模拟高过载(+Gx)环境下猴颞肌肌细胞组织及c-jun蛋白表达的变化。结果:组织病理学观察:实验组和对照组猴颞肌肌细胞无明显变化;免疫组织化学观察:对照组颞肌c-jun呈阴性或弱阳性表达,主要位于肌细胞核,胞质着色不明显。实验组颞肌细胞核c-jun着色明显,呈强阳性表达,不同高过载(+Gx)组之间无明显差别。结论:高过载(+Gx)环境中,猴颞肌细胞c-jun蛋白表达明显增强。Objective： To observe the changes of c-jun expression in the monkey＇s temporalis muscles induced by stress reaction under ＋ Gx loads. Methods ： Nine male Rhesus macaques were randomly divided into four groups according to Gx loads and its lasting time： control group was exposed to ＋ 1 Gx/300 s overloads（ n =2）, and experimental groups 1（n =2）, 2 （n =2） and 3（n =3） were exposed to ＋ 15 Gx/200 s, ＋21 Gx/165 s or ＋21 Gx/140 s respectively. Temporalis muscles tissue was fixed with 40 g/L buffered formaldehyde and embedded in paraffin. Histopathological changes were observed under light microscope. The expression of c-jun was detected by immunohistochemical PicTureTM method in the temporalis muscles, Results： Gross and histological analyses showed that no significant pathological changes in the temporalis muscles in different experimental groups. Immunohistochemical staining demonstrated that c-jun was over-expressed in the nuclei of the muscles in the experimental groups, while negative or slight positive staining was observed in the nuclei of muscles in control animals. There was no obvious difference of c- jun expression in different experimental groups. Conclusion：Overload gravity can induce enhanced expression of c-jun in temporalis muscles of the monkeys received ＋ Gx loads.

关 键 词：高过载(+Gx) 颞肌细胞 c—jun表达 

分 类 号：R855[医药卫生—航空、航天与航海医学]