RP-HPLC法测定烟酸维生素B_6注射液中2组分含量  

Determination the Contents of Two Components in Nicotinic Acid and Vitamin B_6 Injection by HPLC

作　　者：付萍萍[1] 杨开宁 赵志伟[1] 景浩然[1] 

机构地区：[1]保定市药品检验所,河北保定071051 [2]保定市第二中心医院

出　　处：《中国药师》2007年第6期551-552,共2页China Pharmacist

摘　　要：目的:建立HPLC法测定烟酸维生素B_6注射液中烟酸和维生素B_6含量的方法。方法:采用C_(18)(200 mm×4.6 mm,5μm)色谱柱,以0.04%戊烷磺酸钠溶液(用冰醋酸调节pH为3.0±0.05)-甲醇(90:10)为流动相,检测波长为262nm,流速为1.0ml·min^(-1)。结果:烟酸的线性范围为0.04～1.01μg(r=1.000 0,n=7),平均回收率为100.3%(n=9),RSD为0.4%;维生素B_6的线性范围为0.12～3.03μg(r=1.000 0,n=7),平均回收率为100.2%(n=9),RSD为0.5%。结论:本方法快速、准确,可用于烟酸维生素B_6注射液的质量控制。Objective： To establish a RP-HPLC method for determination the contents of nicotinic acid and Vitamin B6 in Nicotinic acid and Vitamin B6 injection, Method： The Hypersil C18 column（200 mm ×4.6 mm,5 μm）was used, the 0.04% Sodium Pentanesulfonate solution（ adjuste pH to 3.0 ± 0. 05 with glacial acetic acid ） -methanol （90： 10）as the mobile phase, the flow rate was 1.0 ml. min^-1. The detection wavelength was at 262 nm. Result： The linear ranges of nicotinic acid and vitamin B6 were at 0.04 - 10 μg（r = 1.000 0） and 0. 12 -3.03 μg（r = 1,000 0）respectively, the average recoveries were 100.3% and 100.2% with RSD 0,4% and 0,5% respectively. Conclusion： The method is quick and accurate. It can be used for quality control of nicotinic acid and vitamin B6 injection.

关 键 词：烟酸 维生素B6 含量测定 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]