心律宁片中苦参碱和氧化苦参碱的含量测定  

Determination of Matrine and Oxymatrine in Xinlii Ning Tablet by HPLC

作　　者：陶平德[1] 蒋平[2] 杨红斌[2] 姚育端[2] 

机构地区：[1]十堰市太和医院,湖北十堰442000 [2]华中科技大学同济医学院附属同济医院

出　　处：《中国药师》2007年第6期554-555,共2页China Pharmacist

摘　　要：目的:建立心律宁片中苦参碱和氧化苦参碱含量的测定方法。方法:色谱柱为Alltima Amino(250mm×4.6mm,5μm),流动相为乙腈-无水乙醇-3%磷酸溶液(80:10:10),检测波长为220nm,流速为1.0ml·min^(-1);柱温:室温。结果:苦参碱线性范围是0.168μg～3.360μg,r=0.999 7,氧化苦参碱线性范围是0.124μg～2.480μg,r=0.999 3,苦参碱及氧化苦参碱的平均回收率分别为101.1%(RSD=1.22%)和100.2%(RSD=1.91%)(n=6)。结论:方法快速简便,重复性良好,可用于测定心律宁片中苦参碱和氧化苦参碱的含量。Objective： To develop a method for the determination of Matrine and Oxymatrine in Xinlü Ning Tablet. Method： Alltima Amino column （ 250 mm × 4.6 mm , 5 μm） was used. The mobile phase was consisted of acetonitrile-ethanol-3% phosphoric acid （80： 10： 10） and the flow rate was 1.0 ml.min^-1. The UV detection wavelength was at 220 nm and the column was kept at room temperature. Result： Good linear relations were achieved. The Matrine concentration ranges of 0. 168 μg - 3.36μg （ r = 0.999 7 ）, and the average recovery were 101.1% （ RSD = 1.22%, n = 6 ）. The Oxymatrine concentration ranges of 0. 124 μg - 2.48 μg （ r = 0.999 3 ）, and the average recovery were 100.2% （ RSD = 1.91%, n = 6）. Conclusion： The method was rapid and reproducible, and was suitable for the determination of the content of Matrine and Oxymatrine in XinlüNing tablet.

关 键 词：心律宁片 苦参碱 氧化苦参碱 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]