IL-6基因启动子区-634C/G多态性与糖尿病肾病的相关性  

The association of IL-6 gene promoter region-643 C/G polymorphism with diabetic nephropathy

作　　者：安新焕[1] 宋滇平[1] 刘华[2] 王玉明[2] 段勇[2] 冯霞[2] 

机构地区：[1]昆明医学院第一附属医院糖尿病科,现在广东省中山市人民医院650032 [2]昆明医学院第一附属医院检验科,650032

出　　处：《中国糖尿病杂志》2007年第5期289-291,共3页Chinese Journal of Diabetes

基　　金：云南省教育厅基金资助项目(04Z021C)

摘　　要：目的探讨白细胞介素6(IL-6)基因启动子区-634C/G多态性与糖尿病肾病(DN)的关系。方法在昆明地区汉族人中,应用PCR-RFLP方法和等位特异PCR(ASPCR)方法,对246例2型糖尿病(T2DM)患者(正常白蛋白尿组88例,微量白蛋白尿组93例,大量白蛋白尿组65例)和101例健康对照者(NC组)的IL-6基因启动子区-634C/G多态性进行检测。结果(1)微量白蛋白尿组、大量白蛋白尿组、微量、大量白蛋白尿组的G/G基因型频率均高于正常白蛋白尿组(P=0.001),大量白蛋白尿组亦高于微量白蛋白尿组(P=0.045)。(2)大量白蛋白尿组和大量、微量白蛋白尿组的G等位基因频率均高于正常白蛋白尿组(P=0.031),大量白蛋白尿组亦高于微量白蛋白尿组(P=0.005)。(3)T2DM组中,GG基因型组UAER明显高于CG、CC基因型组(P<0.01)。(4)Logistic回归分析表明:IL-6基因启动子区-634G/G基因型、病程是DN发生的危险因素。结论在昆明地区汉族人中,IL-6基因启动子区-634G/G基因型可能是DN发生的危险因素,此基因型携带者UAER明显增高;G等位基因可能是DN发展的危险因素之一。Objective To investigate the association of IL-6 gene promoter region -643 C/G polymorphism with diabetic nephropathy. Methods The 246 unrelated Kunming Han people with type 2 diabetes,including 88 cases with normal albuminuria （DN0 group） ,93 cases with microalbuminuria （DN1 group）, 65 cases with macroalbuminuria （DN2 group） and 101 normal controls（NC group）, were enrolled in this study. The polymorphism of IL-6 gene promoter region-634C/G were detected by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） and allele specific PCR（ASPCR） methods. Results （1） The frequencies of G/G genotype in DN1.DN2 and DN（DN1-{--DN2） groups were significantly higher than in DN0 group, and there were significant differences in genotype frequencies between DN2 and DN1 groups. （2） The frequencies of G allele in DN2 and DN（DN1＋DN2）groups were significantly higher than in DN0 group,and there was significant difference in G allele frequency between DN2 and DN1 groups （3） The urinary albumin excretion rate in G/G genotype group was significantly higher than in C/G and G/G genotype groups. There were no statistic differences in other clinical data in type 2 diabetes with different genotypes. （4） Logistic regression analysis showed that IL-6 gene promoter region -643G/G genotype and diabetic duration were independent risk factors for DN development. Conclusions The IL-6 promoter region -643G/G genotype may be a genetic risk factor for DN development, and its urinary albumin excretion rate is significantly higher than the others; IL-6 -634G allele may be one of the risk factors for DN progression.

关 键 词：白细胞介素6 基因 启动子区 多态性 糖尿病肾病 

分 类 号：R587.2[医药卫生—内分泌]