pEGFP-P21-WT及pEGFP-P21-S145A融合表达载体在小鼠卵母细胞中的定位  

The Localization of P21 and P21 S145A Mutant in Mouse Oocytes

作　　者：武迪迪[1] 韩晓曦[1] 刘莹[1] 张杰[1] 具英花[1] 李雪松[1] 于秉治[1] 

机构地区：[1]中国医科大学基础医学院生物化学与分子生物学教研室,沈阳市110001

出　　处：《医学分子生物学杂志》2007年第3期243-244,252,共3页Journal of Medical Molecular Biology

基　　金：国家自然科学基金(No.39730460);辽宁省博士启动基金(No.20041043)~~

摘　　要：目的通过构建pEGFP-P21-WT及pEGFP-P21-S145A融合表达载体,确定在小鼠卵母细胞中的定位。方法采用显微注射方法将融合表达载体导入小鼠卵母细胞中,荧光镜下直接观察P21及突变体的细胞定位。结果pEGFP-P21-WT注射后,荧光分布集中于核中,而pEGFP-P21-S145A注射后,荧光信号主要分布于胞浆中,对照组(注射pEGFP-C3)荧光信号均匀分布于整个细胞内。结论P21蛋白145位突变后影响了P21蛋白的细胞定位。Objective To investigate the localization of P21 and P21S145A mutant in mouse oocytes. Methods Fusion expression vector pEGFP-P21-WT and pEGFP-P21 -S145A were constructed and microinjected to mouse oocytes. Localization was observed under fluorescent microscope. Result Green fluorescence signals were detected in nuclei after microinjecting with pEGFP-P21-WT while they were in cytoplasm after microinjecting with pEGFP-P21-S145A. Conclusion The cell localization of P21 was altered by microinjection of pEGFP-P21-S145A vector.

关 键 词：P21 细胞周期 小鼠卵母细胞 

分 类 号：Q132.7[生物学—普通生物学]