Prp8蛋白在前体mRNA剪接中的作用  

The Role of Prp8 Protein in the Pre-mRNA Splicing

作　　者：洪敬欣[1] 姚智[1] 杨洁[1] 

机构地区：[1]天津医科大学免疫学教研室,天津市300070

出　　处：《医学分子生物学杂志》2007年第3期253-256,共4页Journal of Medical Molecular Biology

基　　金：国家自然科学基金(No.30670441;30300070);天津市科委应用基础研究重点项目(No.043802811);国家教育部新世纪人才支持计划(No.NCET-04-0245);高等学校博士学科点转项基金(No.20040062003)~~

摘　　要：前体mRNA的剪接是基因表达的关键一步,发生在蛋白质的转录之后与合成之前。在前体mR- NA剪接加工过程中需要将转录本中的内含子切除,因为它会干扰基因的转录。前体mRNA的剪接发生在细胞核中,是在一个大的RNA与蛋白质的复合物即剪接体的催化下完成的。Prp8(precursor mRNA process- ing)是参与前体mRNA剪接的最大的蛋白,其序列从酵母到人类是高度保守的。Prp8同时也是细胞核内一个最重要的剪接因子。在剪接过程中,Prp8组成剪接体的催化中心。有人推断Prp8是剪接体的支架蛋白,很可能在催化中心起到锚定RNA的作用,同时也调节着激活剪接体所必需的构象变化。Prp8还与色素性视网膜炎的发生密切相关。Pre-messenger RNA （pre-mRNA） splicing is a critical step in gene expression, which takes place between transcription and protein synthesis. Pre-mRNA splicing removes sequence （introns） that will otherwise disrupt the gene expression process. This process takes place in the nuclei, and is catalyzed by a large RNA-protein complex called spliceosome. Prp8 （precursor mRNA processing） is the largest protein involved in nuclear pre-mRNA splicing, and its sequence is highly conservative from yeast to human. It is also one of the most important splicing factors in nuclei. During splicing, it serves as the catalytic center of the spliceosome. It has been proposed that it acts as a protein scaffold in the spliceosome, possibly anchoring ranks in the catalytic center and regulating conformational changes that are important for activation of the spliceosome. Prp8 is also closely related to the retinitis pigmentosa.

关 键 词：Prp8蛋白 前体MRNA 支架蛋白 

分 类 号：Q522.2[生物学—生物化学]