通脉益智胶囊增加ApoE基因敲除小鼠易损动脉粥样硬化斑块稳定性的作用  

Effect of Tongmaiyizhi Capsules on stability of vulnerable atherosclerotic plaque in ApoE-gene knockout mice

作　　者：路永刚[1] 张铁忠[1] 程文丽[2] 向青[2] 徐梅[2] 徐波[2] 陈旺[2] 李鸿[2] 郭健[2] 

机构地区：[1]中日友好医院中医老年病科,北京100029 [2]中日友好医院临床研究所

出　　处：《北京中医药大学学报》2007年第5期329-332,F0003,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：中日友好医院院内课题(No.20053079)

摘　　要：目的观察通脉益智胶囊对ApoE基因敲除小鼠(ApoE^(-/-)小鼠)无名动脉易损动脉粥样硬化(AS)斑块是否具有稳定作用。方法8周龄的ApoE^(-/-)小鼠给予高脂饲料22周后,无名动脉已经形成易损AS斑块,将小鼠随机分为3组:通脉益智胶囊组、辛伐他汀组和模型组,另设6只正常C57BL／6小鼠作为对照组,分别给予720mg／(kg·d)通脉益智胶囊、2.8mg／(kg·d)辛伐他汀及生理盐水,8周后用免疫组化、Movat Pentachrome以及天狼星红染色观察各组AS斑块的形态变化。结果与模型组相比较,通脉益智胶囊组可显著降低AS斑块的面积、增加斑块胶原组织的含量(P＜0.01、P＜0.05),辛伐他汀组可显著增强AS斑块纤维帽的连续性、增加纤维帽的厚度(P＜0.05)。结论通脉益智胶囊可通过减小AS面积以及增加斑块内部胶原组织,辛伐他汀可通过稳定斑块表面的纤维帽,分别达到稳定AS斑块的作用。Objective To investigate the effect of Tongmaiyizhi Capsules and simvastatin on advanced unstable atherosclerotic lesions within the innominate artery of ApoeE-gene knockout mice. Methods After fed with high fat diet for 22 weeks, 8 week-old ApoeE-gene knockout mice were randomly divided into three groups, model group , Tongmaiyizhi group and simvastatin group, which were administered with Tongmaiyizhi Capsules （720 mg/kg/d） , simvastatin （2.8 mg/kg/d） and SodiumChloride for 8 weeks respectively. Six normal C57BL/6 mice were set as the control group and were also administered with SodiumChloride for 8 weeks. Immunohistochemistry , Movat Pentachrome and Sirius Red-staining methods were used to evaluate the histomorphological changes of the plaque. Results Compared with the model group, the average area of AS lesion decreased and the content of collagen were significantly increased （ P 〈0.01 ,P 〈 0.05 ）in the Tongmaiyizhi Capsules group; the thickness, integrity of the fibrous cap were enhanced in the simvastatin group （ P 〈 0.05, P 〈 0.05 ）. Conclusions Tongmaiyizhi Capsules can stabilize the vulnerable atherosclerotic plaque through decreasing AS area and increasing collagen tissue; simvastatin can stabilize the AS plaque through stabilizing the fibrous cap on plaque surface.

关 键 词：易损斑块 动脉粥样硬化斑块稳定性 通脉益智胶囊 辛伐他汀 APOE基因敲除小鼠 

分 类 号：R285.5[医药卫生—中药学]