乙酰胆碱对非洲爪蟾卵母细胞ATP-激活电流的调制作用  

Modulation of ATP-activated current by acetylcholine in Xenopus oocytes

作　　者：程剑侠[1] 王照宇[1] 朱凡[1] 张玉芹[1] 

机构地区：[1]武汉科技大学医学院,湖北武汉430080

出　　处：《武汉科技大学学报》2007年第3期305-307,共3页Journal of Wuhan University of Science and Technology

基　　金：湖北省自然科学基金资助项目(2004ABA152);湖北省教育厅资助项目(2006B079)

摘　　要：研究乙酰胆碱对爪蟾卵母细胞上ATP激活电流的影响及其机理。用双电极电压钳技术记录爪蟾卵母细胞的细胞外液加入乙酰胆碱对ATP激活电流(IATP)的影响。结果显示,乙酰胆碱对大多数细胞IATP具有增强作用,而对少数细胞IATP具有抑制作用,且均呈现浓度依赖性;乙酰胆碱对IATP的调制作用是通过毒蕈碱样乙酰胆碱受体(M受体)激活实现的。To investigate the modulatory effect of acetylcholine （Ach） on ATP-activated currents （IATP） of Xenopus oocytes, two electrode voltage-clamp technique is used to record the IATP in healthy Xenopus oocytes by applying Ach in extracellular fluid. The experimental result shows that in the most cells ATP-activated currents recorded are enhanced, while in a few cells they are inhibited, both of which are in a concentration-dependent manner. The modulatory effect of Ach on the IATP may be achieved by activating muscarinic receptor （M receptor）.

关 键 词：M受体 爪蟾卵母细胞 ATP-激活电流 双极电压钳技术 

分 类 号：R338.1[医药卫生—人体生理学]