马铃薯块茎中总RNA的快速提取  

Quick Extraction of Total RNA from Potato Tuber

作　　者：孙琦[1] 张春庆[2] 孟昭东[1] 张发军[1] 丁照华[1] 穆春华[1] 李文才[1] 

机构地区：[1]山东省农业科学院玉米研究所,济南250100 [2]山东农业大学农学院,泰安271018

出　　处：《生物技术通报》2007年第3期152-154,共3页Biotechnology Bulletin

摘　　要：马铃薯块茎中由于含有大量的多糖和酚类物质,因此难以从中提取RNA。本实验采用CTAB法从马铃薯块茎中提取了总RNA,并进行了马铃薯病毒的检验。结果表明:得到的RNA完整性好,A260/A280在1.7～2.0范围内,纯度也比较高。用RT-PCR方法进行马铃薯Y病毒的检验,得到了一条与预计扩增长度相同的一条带,并检验出枣庄地区马铃薯Y病毒的感染率为75%。RNA was difficult to extract from potato tubers because of a great deal of amylase and phenolic compound in them.The total RNA was isolated from potato tubers with CTAB method and used for detecting potato virus.The extracted total RNA was of high integrality and the A260/A280 ratio of each sample was between 1.7 and 2,0. The total RNA could be used to detect potato virus with RT-PCR,

关 键 词：马铃薯块茎 总RNA提取 CTAB 

分 类 号：S532[农业科学—作物学]