康欣胶囊及其拆方对肾虚血瘀型大鼠神经细胞的保护作用  

Effect of Kangxin Capsule and Its Different Compositions on Nerve Cells of Rats with Kidney Deficiency and Blood Stasis

作　　者：蔡晶[1] 杜建[1] 刘锡棐[2] 陈旭征[1] 

机构地区：[1]福建中医学院,福州市五四路282号350003 [2]南京军区南京总医院神经内科

出　　处：《中医杂志》2007年第5期453-456,共4页Journal of Traditional Chinese Medicine

基　　金：福建省卫生厅重大中医药项目(2003-101);福建省青年科技人才创新基金资助项目(2002J055)

摘　　要：目的探讨康欣胶囊全方及其补肾、健脾、养血活血3组拆方对肾虚血瘀型血管性痴呆(VD)大鼠神经细胞的影响。方法采用双侧椎动脉永久性封闭和摘除单侧性器官建立肾虚血瘀型VD模型大鼠,分别给予康欣胶囊全方(全方组)、康欣胶囊补肾方(补肾组)、康欣胶囊健脾方(健脾组)、康欣胶囊养血活血方(养血活血组)、阳性对照药喜得镇(西药组)和蒸馏水(模型组)。结果全方组大鼠空间探索能力显著优于其他组(P<0.01);全方组海马神经细胞器及细胞核固缩情况轻于其他各组;全方组能增加海马乙酰胆碱含量(P<0.01),提高VD大鼠海马酪氨酸激酶A、核转录因子、原癌基因C-fos、神经生长因子受体的表达(P<0.01),降低胶质纤维酸性蛋白的表达(P<0.05)。结论康欣胶囊全方对肾虚血瘀型血管性痴呆大鼠神经细胞具有保护作用。Objective To study Kangxin Capsule and its kidney-supplementing, spleen-fortifying and blood-nourishing-quickening compositions on nerve cells of vascular dementia （VD） rats with kidney deficiency and blood stasis. Methods Hibateral vertebral arteries were permanently blocked and unilateral genital was extirpated to establish VD rat models with kidney deficiency and blood stasis. Hydergine, distilled water, Kangxin Capsule and its kidney-supplementing, spleen-fortifying and blood-nourishing-quickening compositions were given to rat models respectively. Results Kangxin Capsule could improve spatial exploration ability of rats, relieve pycnosis of nerve cells in hippocampus, increase the content of acetylcholine, up-regulate the expression of tyrosine kinase A, nuclear transcription factor-κB, C-fos and Nerve growth factor receptor and down-regulate the expression of glial fibrillary acidic protein significantly as compared with other groups （P〈0. 01 or P〈0.05）. Conclusion Kangxin Capsule has a protective effect on nerve cells of VD rats with kidney deficiency and blood stasis.

关 键 词：康欣胶囊 肾虚血瘀 血管性痴呆 酪氨酸激酶A 核转录因子 C-fos 神经生长因子受体 胶质纤维酸性蛋白 

分 类 号：R285.5[医药卫生—中药学]