含硫树脂的制备及在谷胱甘肽分离中的应用  

The preparation of resin containing sulf-group and their application in glutathione separation

作　　者：赵睿[1] 王满意[1] 周鑫[1] 谭天伟[1] 

机构地区：[1]北京化工大学生命科学与技术学院

出　　处：《北京化工大学学报（自然科学版）》2007年第3期304-307,共4页Journal of Beijing University of Chemical Technology(Natural Science Edition)

基　　金：国家自然科学基金(20325622/20576013/50373003)

摘　　要：利用大孔苯乙烯树脂改性合成含硫树脂,考察了修饰条件对谷胱甘肽吸附的影响。并对其在谷胱甘肽分离中的应用进行了研究。结果表明采用pH3.0的缓冲液进行吸附,自制的含硫树脂最大吸附容量可达22 mg/g湿树脂,选择质量浓度为0.5%的NaOH溶液洗脱GSH,解吸率可达70%以上。采用真实发酵液进行分离纯化,收率为35%,纯度可达90%以上。Chloromethylated styrene-divinylbenzene resin has been modified with thiourea under a variety of conditions and the adsorption and desorption of glutathione on the modified resins has been investigated. The optimal pH for adsorption was found to be 3.0, giving a maximum adsorption capacity of glutathione of 22 mg/g wet resin. Recovery of glutathione reached 70 % when 0.5 96 - 1.5 % NaOH solution was employed for desorption. Chromatography experiments indicated that GSH could be successfully purified from clarified broth, and that the resins have potential applications in GSH separation.

关 键 词：含硫树脂 修饰 谷胱甘肽 

分 类 号：O658[理学—分析化学]