地塞米松及葡萄糖对缺血缺氧性脑损伤新生大鼠血糖和脑ATP生成率及ATP酶分解活性的影响  

Changes of blood sugar,mitochondrial ATP production rate and ATP synthase activity in dexamethasone and glucose treated neonatal rat after hypoxic-ischemic brain damage

作　　者：陈凌梅[1] 李炜如[2] 

机构地区：[1]成都市儿童医院,成都610017 [2]四川大学华西第二医院儿科

出　　处：《中华妇幼临床医学杂志（电子版）》2007年第3期145-148,共4页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

摘　　要：目的缺血缺氧性脑损伤(HIBD)的发生与脑组织能量耗竭、神经元结构和功能障碍有关。既往的研究显示,地塞米松预处理可产生脑保护作用。但关于缺氧缺血后,给予地塞米松及葡萄糖治疗对脑能量代谢影响的研究不多。本研究通过测定血糖、脑组织ATP生成率及ATP酶分解活性,了解地塞米松及葡萄糖治疗对缺氧缺血新生大鼠能量代谢的影响。方法将7d龄SD大鼠60只随机分成缺血缺氧性脑损伤组(HIBD组)、地塞米松治疗组及葡萄糖治疗组。3组动物经缺氧缺血处理后,HIBD组不做治疗、地塞米松组及葡萄糖治疗组分别以地塞米松及葡萄糖进行治疗。检测0h,24h,48h,72h实验大鼠的血糖、脑线粒体ATP生成率及ATP酶分解活性变化。结果1缺氧缺血处理后,大鼠血糖、脑组织线粒体ATP生成率及ATP酶分解活性下降;2地塞米松治疗组ATP生成率24h、48h时较HIBD组高;ATP酶分解活性24h时较HIBD组高;3葡萄糖治疗组脑组织线粒体ATP生成率48h时较HIBD组高;472h时3组动物ATP生成率及ATP酶分解活性差异无显著意义;5地塞米松治疗组及葡萄糖治疗组的血糖在24h时较HIBD组高;648h及72h时,3组血糖值差异无显著意义。结论地塞米松及葡萄糖可在短期内改善缺血缺氧新生大鼠脑组织能量供应,并对血糖产生影响。Objective To study the changes in blood sugar, mitochondrial ATP production rate and ATP synthase activity in dexamethasone treated and in glucose treated neonatal rats after hypoxic-ischemic brain damage. Methods 7 days old rats were randomly assigned to HIBD group, dexamethasone treated group and glucose treated group. Had been made cerebral hypoxic-ischemic, the HIBD animals were divided into 0 h, 24 h, 48 h and 72 h groups. the dexamethasone treated animals and glucose treated animals were divided into 24 h, 48 h and 72 h groups and treated with dexamethasone and glucose separately. Then we examined changes in blood sugar, mitochondrial ATP production rate and ATP synthase activity. Results （1） After hypoxic-ischemic, the rats＇ blood sugar, mitochondrial ATP production rate and ATP synthase activity were decreased. （2）In dexamethasone treated group the ATP production rate at 24 h and 48 h, ATP synthase activity at 24 h were higher than HIBD group. （3）In glucose treated group, the ATP production rate at 48 h were higher than HIBD group. （4） At 72 h, there were no differences in mitochondrial ATP production rate and ATP synthase activity between 3 groups. （5） The blood sugar in dexamethasone treated group and in glucose treated group were higher than HIBD group at 24 h. （6） At 48 h and 72 h, there were no differences in blood sugar between 3 groups. Conclusion Dexamethasone and glucose improved HIBD rats＇ cerebral energy metabolize in a short term and affected blood glucose.

关 键 词：缺氧缺血性脑损伤 ATP ATP合成酶 血糖 

分 类 号：R722.1[医药卫生—儿科]