Interventional effect of magnesium sulfate on nitric oxide synthase activity after acute craniocerebral injury  

作　　者：Ximin Yang Jiangong Zhu Zongchun Tang 

机构地区：[1]The Third Hospital of Chinese PLA, Baoji 721004, Shaanxi Province, China

出　　处：《Neural Regeneration Research》2007年第4期251-253,共3页中国神经再生研究（英文版）

摘　　要：BACKGROUND： Abnormal changes in magnesium ion are closely related to cerebral injury. At present, some evidence indicates that magnesium reagent can improve nerve function and prognosis of patients with cerebral injury. OBJECTIVE： To observe the effect of magnesium sulfate on changes in nitric oxide synthase （NOS） activity in brain tissue of rats with acute craniocerebral injury. DESIGN： Completely randomized grouping design and randomly controlled study. SETTING： Laboratory of Neurosurgery, the Third Hospital of Chinese PLA. MATERIALS： Fifty-four male SD rats of clean grade and weighing 220 - 250 g were randomly divided into normal control group （n =6）, cerebral injury group （n =24） and magnesium sulfate group （n =24）. Especially, rats in cerebral injury group and magnesium sulfate group were equally divided into four subgroups and observed at 0.5, 2, 6 and 24 hours after model establishment. A solution of 125 g/L of magnesium sulfate was provided by the Seventh Pharmaceutical Factory of Wuxi and the NOS assay kit by Nanjing Jiancheng Bioengineering Institute. METHODS： The experiment was carried out in the Institute of Neurosurgery, the Third Hospital of Chinese PLA from August 2000 to August 2002. ① Rats in the cerebral injury group and magnesium sulfate group were anesthetized to establish cerebral injury models based on modified Feeney technique; magnesium sulfate group were intraperitoneally injected 600 mg/kg magnesium sulfate （125 g/L）, but rats in the normal control group remained untreated. ② At 0.5, 2, 6 and 24 hours after cerebral injury, rats in cerebral injury group and magnesium sulfate group were decapitated and brains were dissected. Cerebral cortex of rats in cerebral injury group was selected for NOS assay; in addition, at 0.5 hour after cerebral injury, a portion of the parietal lobe was selected from the brains of rats in the normal control group. Brain samples were homogenized, the homogenated centrifuged and the supernatants were used to measure NOS activiBACKGROUND： Abnormal changes in magnesium ion are closely related to cerebral injury. At present, some evidence indicates that magnesium reagent can improve nerve function and prognosis of patients with cerebral injury. OBJECTIVE： To observe the effect of magnesium sulfate on changes in nitric oxide synthase （NOS） activity in brain tissue of rats with acute craniocerebral injury. DESIGN： Completely randomized grouping design and randomly controlled study. SETTING： Laboratory of Neurosurgery, the Third Hospital of Chinese PLA. MATERIALS： Fifty-four male SD rats of clean grade and weighing 220 - 250 g were randomly divided into normal control group （n =6）, cerebral injury group （n =24） and magnesium sulfate group （n =24）. Especially, rats in cerebral injury group and magnesium sulfate group were equally divided into four subgroups and observed at 0.5, 2, 6 and 24 hours after model establishment. A solution of 125 g/L of magnesium sulfate was provided by the Seventh Pharmaceutical Factory of Wuxi and the NOS assay kit by Nanjing Jiancheng Bioengineering Institute. METHODS： The experiment was carried out in the Institute of Neurosurgery, the Third Hospital of Chinese PLA from August 2000 to August 2002. ① Rats in the cerebral injury group and magnesium sulfate group were anesthetized to establish cerebral injury models based on modified Feeney technique; magnesium sulfate group were intraperitoneally injected 600 mg/kg magnesium sulfate （125 g/L）, but rats in the normal control group remained untreated. ② At 0.5, 2, 6 and 24 hours after cerebral injury, rats in cerebral injury group and magnesium sulfate group were decapitated and brains were dissected. Cerebral cortex of rats in cerebral injury group was selected for NOS assay; in addition, at 0.5 hour after cerebral injury, a portion of the parietal lobe was selected from the brains of rats in the normal control group. Brain samples were homogenized, the homogenated centrifuged and the supernatants were used to measure NOS activi

关 键 词：brain injuries nitric-oxide synthase magnesium sulfate 

分 类 号：R651.15[医药卫生—外科学]