血液与非血液灌流行缺血预处理鼠心的电生理实验研究  

Cardiac Electrophysiological Experimental Studies on Ischemia Preconditioned Rat Heart Perfused with Blood and Krebs-Henseleit Solution

作　　者：沈岳良[1,2] 夏强[1,2] 黄德明[1,2] 陆源[1,2] 谢丽平 罗建根[1,2] 

机构地区：[1]浙江医科大学生理教研室 [2]香港大学生理系

出　　处：《中华胸心血管外科杂志》1997年第1期53-55,共3页Chinese Journal of Thoracic and Cardiovascular Surgery

基　　金：卫生部和浙江省卫生厅科研基金

摘　　要：本实验研究血液与非血液灌流条件下缺血预处理（ＩＰ）对心肌的保护作用。应用离体大鼠Ｌａｎｇｅｎｄｏｒｆ灌流模型，分血液灌流ＩＰ组、非血液（ＫｒｅｂｓＨｅｎｓｅｌｅｉｔ液，ＫＨ液）灌流ＩＰ组及相应的对照组。测量心肌电生理特性等指标。结果与ＫＨ液灌流心脏相似，用血液灌流的大鼠心脏经ＩＰ后，在随后的停灌和复灌期间，心室颤动阈升高，心律失常发生率明显降低。实验结论为在用血液灌流离体大鼠心脏模型上，ＩＰ对心肌的保护作用与用生理溶液灌流的模型基本相同。Objective and Method:The aim of the present study was to determine the cardiac electrophysiological responses of ischemia preconditioned (IP) isolated rat heart separately perfused with blood and Krebs-Henseleit solution using Langendorff apparatus.The investigations were divided into four groups:group A:Blood perfusion+IP;group B:K H solution perfusion+IP;group C:control of group A;group D:control of group B.Determinations of diastolic threshold (DT),effective refractory period (ERP),ventricular fibrillation threshold (VFT) were performed and EKG recorded at different intervals.Changes of DT and ERP showed no statistical siginficance.Results:During the period of ischemia and reperfusion following ischemia preconditioning,ventricular fibrillation thresholds were markedly increased and incidence of arrhythmias was significantly reduced in both blood perfuseds and Krebs-Henseleit solution perfused ones as compared with the values of their control groups.However,there was significant difference between group A and group B.Conclusion:The results indicate the cardiac protective effects of ischemia preconditioning on isolated rat hearts were similar in both blood perfused and crystalloid solution perfused groups.

关 键 词：缺血预处理 心律失常 血液灌流 

分 类 号：R654.2[医药卫生—外科学]