不同嗜性HIV复制与树突状细胞成熟状态关系的研究  

Study on the relationship between replication of HIV-1 with different tropism and the developmental state of dendritic cells

作　　者：耿文清[1] 尚红[1] 姜拥军[1] 王亚男[1] 苏艳丽[1] 张子宁[1] 韩晓旭[1] 刁莹莹[1] 代娣[1] 刘静[1] 张旻[1] 

机构地区：[1]中国医科大学附属第一医院,卫生部艾滋病免疫学重点实验室,沈阳110001

出　　处：《中华微生物学和免疫学杂志》2007年第5期405-409,共5页Chinese Journal of Microbiology and Immunology

基　　金：基金项目：教育部博士点专项（20040159005）;辽宁省优秀青年科研人才培养基金资助项目（3040011）;国家重点基础研究发展计划973计划（2006CB504206）

摘　　要：目的 探讨不同嗜性HIV-1在人树突状细胞（dendriticcell，DC）内复制与DC成熟状态的关系。方法 采用MACS磁珠分选法纯化出CD14^＋细胞，加入粒细胞-巨噬细胞集落刺激因子（GM-CSF）和白细胞介素-4体外培养7d，得到未成熟DC；再加入肿瘤坏死因子-α继续培养3d，得到成熟DC。感染不同嗜性HIV-1病毒株后，检测培养上清p24含量，观察HIV-1复制能力。结果 受T嗜性HIV-1攻击后，未成熟DC及成熟DC培养上清p24抗原含量随培养时间延长无明显增加。受M嗜性HIV-1攻击后，未成熟DC培养上清p24抗原含量随培养时间延长明显增加，成熟DC培养上清p24抗原含量不随培养时间延长明显增加。结论 T嗜性HIV-1不能在未成熟DC和成熟DC内复制，M嗜性HIV-1能在未成熟DC内复制但不能在成熟DC内复制，提示HIV-1能否在DC内复制与HIV-1嗜性和DC成熟状态有关。Objective To detemine the relationship between replication of HIV-1 primary isolates with M-tropic or T-tropic and differentiation state of dendritic cells（DC）. Methods CD14^＋ cells were isolated from peripheral blood mononuclear cells of healthy donors. The purified CD14^＋ cells were cultured in the presence of granulocytemacrophage colony-stimulating factor（GM-CSF） and intedukin-4 （IL-4） for 7 days. Cells were then cultured for fur- ther 3 days in the presence of TNF-α to get mature DC. After infection with different tropic HIV- 1 primary isolates, the level of p24 antigen in the culture supematant was detected to observe the dynamic alteration of HIV- 1 replication. Results The level of p24 antigen in culture supematant of immature DC and mature DC was not significantly increased after exposure to T-tropic HIV-1. The level of p24 antigen in culture supematant of immature DC increased significantly after exposure to M-tropic HIV-1, but rather than mature DC. Conclusion T-tropic HIV-1 do not replicate in immature and mature DC. M-tropic HIV-1 could replicate in immature DC, but not in mature DC. The data indicated that whether HIV-1 replicate in DC depended on the tropism of HIV-1 and the differential stage of DC.

关 键 词：H1V-1 树突状细胞 M嗜性毒株 T嗜性毒株 

分 类 号：R686[医药卫生—骨科学]