嗅鞘细胞移植和左旋硝基精氨酸对大鼠脊髓损伤后背核神经元存活及其轴突再生的影响  

EFFECTS OF TRANSPLANTED OLFACTORY ENSHEATHING CELLS AND L-NNA ON NEURONAL SURVIVAL AND AXON REGENERATION IN CLARKE'S NUCLEUS AFTER SPINAL CORD HEMISECTION OF RAT

作　　者：张静[1] 曾园山[1] 张伟 陈穗君[1] 

机构地区：[1]中山大学中山医学院组织胚胎学教研室神经科学研究室,广州510080

出　　处：《解剖学报》2007年第3期271-277,共7页Acta Anatomica Sinica

摘　　要：目的探讨嗅鞘细胞(OECs)移植和一氧化氮合酶(NOS)抑制剂左旋硝基精氨酸(L-NNA)对大鼠脊髓损伤后,背核神经元存活及其轴突再生的影响。方法将20只大鼠行脊髓半横断术后分为对照组、OECs组、L-NNA组和OECs+L-NNA组。OECs+L-NNA组在脊髓损伤处移植嗅鞘细胞,并注射L-NNA。术后30d时,应用NADPH酶组织化学、免疫组织化学、中性红染色、HE染色和荧光金逆行标记等方法,观察4组大鼠L1背核神经元存活及其轴突再生情况。结果1.OECs组、L-NNA组L1背核神经元存活数量均高于对照组,且分类计数显示,这两组对脊髓背核大、中、小神经元的存活均有促进作用,而OECs+L-NNA组的促存活作用最显著。2.OECs组和OECs+L-NNA组脊髓损伤处可见再生的轴突,且L1背核可观察到被荧光金逆行标记的神经元胞体。3.与对照组比较,OECs组L1背核NOS阳性神经元增加,L-NNA组NOS阳性神经元减少,OECs+L-NNA组NOS阳性神经元数量差异不明显。结论嗅鞘细胞移植和L-NNA均可促进脊髓受损伤背核神经元的存活,两者联合应用可更好地促进受损伤的背核神经元存活及其轴突再生。Objective To investigate the effects of transplanted olfactory ensheathing cells （OECs）and L-NNA on neuronal survival and axon regeneration in Clarke＇s nucleus （CN） after spinal cord hemiseetion of rats. Methods Twenty rats were divided into control group, L-NNA group, OECs group, and OECs ＋ L-NNA group after spinal cord bemisection. OECs were transplanted into the injured site of spinal cord and L-NNA was injected in OECs ＋ L-NNA group of rats. Thirty days after operation, NADPH enzyme histoehemistry, immunohistochemistry, neutral red staining, HE staining and fluorogold retrogradatie labeling were applied to determine the survival and axon regeneration of neurons at L1 CN in 4 groups of rats. Results 1. The number of neuronal survivals at L1 CN in OECs group and L-NNA group was higher than that in control group. The effect of promoting the survival of big, middle and small neurons at CN of spinal cord in OECs group and L-NNA group was shown by differential counts. The effect of promoting neuronal survival was the most significant in OECs ＋ L-NNA group. 2. The regenerated axons were observed at the injured site of spinal cord and the neuron bodies labeled by fluorogold retrogradation were found at L1 CN in OECs group and OECs ＋ L-NNA group. 3. Compared with those in control group, nitric oxide synthase （NOS） positive neurons were increased in OECs group, decreased in L-NNA group, and were no difference in OECs ＋ L-NNA group. Conclusion Transplanted OECs or L-NNA may facilitate the survival of injured CN neurons of spinal cord. The combination of transplanted OECs and L-NNA can preferably promote the survival and axon regeneration of injured CN neurons.

关 键 词：嗅鞘细胞 左旋硝基精氨酸 脊髓半横断 神经元存活 轴突再生 背核 免疫组织化学 大鼠 

分 类 号：R338.2[医药卫生—人体生理学]