侧脑室注射链脲佐菌素对大鼠海马突触相关蛋白PSD-95和Shank 1表达的影响  

EFFECTS OF INTRACEREBROVENTRICULAR ADMINISTRATION OF STREPTOZOTOCIN ON THE EXPRESSION OF PSD-95 AND Shank 1 IN RATS

作　　者：陆珊[1] 雷亚平[1] 崔艳君[2] 王蓬文[1] 王蓉[3] 盛树力[3] 田金洲[1] 

机构地区：[1]北京中医药大学东直门医院 [2]首都医科大学基础医学院病理学教研室,北京100069 [3]教育部神经变性病学重点实验室,北京100053

出　　处：《解剖学报》2007年第3期278-281,共4页Acta Anatomica Sinica

基　　金：国家重点基础研究发展计划(973计划)课题资助(2003CB517104)

摘　　要：目的观察侧脑室注射链脲佐菌素对大鼠脑PSD-95和Shank1表达的影响。方法将大鼠随机分为正常对照组和模型组,模型组大鼠双侧侧脑室注射链脲佐菌素3mg/kg,第3d重复此剂量;对照组以人工脑脊液代替链脲佐菌素。21d后,取大鼠海马,用免疫组织化学和Western blotting方法观察突触相关蛋白PSD-95和Shank1的表达。结果与对照组相比,模型组大鼠海马PSD-95和Shank1阳性细胞明显减少,PSD95和Shank1蛋白表达降低。结论侧脑室注射链脲佐菌素使海马PSD-95和Shank1表达减少,干扰了神经元突触信号传导。Objective To investigate the effects of intracerebroventricular（ICV） administration of streptozotocin （STZ） on expression of PSD-95 and Shankl in rat hippocampus. Methods A total of 30 rats were randomly divided into normal control group and model group. The rats in the model group received intracerebroventricular injection of STZ bilaterally with a dose of 3mg/kg and the injection was repeated on the third day. The rats in normal control group received the same treatment as the first group except for STZ injection. After 21 days, the brains of the rats were taken, frozen sections of brain were prepared, and then PSD-95 and Shankl were stained with immunohistochemistry. Furthermore, the contents of PSD-95 and Shankl were detected by Western blotting. Results After ICV injection of STZ, the expression of PSD-95 and Shankl positive cells and the contents of PSD-95 and Shankl in hippocampus both decreased. Conclusion ICV injection of STZ can lead to the decrease of the expression of PSD-95 and Shankl, indicating it may damage the neuron＇s synaptic signal transduction.

关 键 词：链脲佐菌素 侧脑室注射 PSD-95 Shank1 免疫组织化学 免疫印迹 大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]