尼古丁对大鼠中脑α-7型神经烟碱胆碱能受体表达的影响  

REGULATION OF α-7 NEURONAL NICOTINIC ACETYLCHOLINE RECEPTORS BY NICOTINE IN MIDBRAIN OF RATS

作　　者：赵家伟[1] 翟秀岩[1] 

机构地区：[1]中国医科大学基础医学院发育生物学教研室,沈阳110001

出　　处：《解剖学报》2007年第3期291-295,共5页Acta Anatomica Sinica

摘　　要：目的观察大鼠暴露尼古丁对中脑腹侧背盖区(VTA)、黑质(SN)和大脑皮质α-7型神经烟碱胆碱能受体(α-7-nAChRs)表达的影响。方法建立暴露尼古丁的大鼠动物模型,应用免疫组织化学法、原位杂交法分别观察VTA、SN和大脑皮质α-7-nAChRs蛋白和mRNA表达的变化,用Western blotting方法分析尼古丁作用后PC12细胞α-7-nAChRs表达的变化。结果尼古丁上调大脑皮质α-7-nAChRs蛋白表达,也上调VTA和SNα-7-nAChRsmRNA的表达。在PC12细胞中,α-7-nAChRs表达随着尼古丁作用时间和剂量的增加而增加。结论尼古丁在转录水平上调VTA和SNα-7-nAChRs的表达,在翻译水平上调大脑皮质α-7-nAChRs的表达。Objective To observe the regulation of α-7 neuronal nicotinic acetylcholine receptors （α-7-nAChRs） by nicotine in the ventral tegmental area（VTA）, the substantia nigra （SN） and the cortex of the rat. Methods Nicotine exposed animal models were established. The changes of α-7-nAChRs proteins and mRNA were observed by immunohistochemistry and in situ hybridization in VTA, SN and cortex, and the expression of α-7-nAChRs proteins by Western blotting in PC12 cells. Results The expression of α-7-nAChRs proteins in cortex and mRNA in VTA and SN were upregulated by nicotine, The expressions of α-7-nAChRs in PC12 cells were in proportion to and dependent on the time for nicotine to function and the dose. Conclusion Both α-7-nAChRs mRNA in VTA and SN in transcriptional mechanism and α-7-nAChRs protein in the cortex at the translation level were upregulated.

关 键 词：尼古丁 α-7型神经烟碱胆碱能受体 腹侧被盖区 黑质 PC12细胞 原位杂交 免疫印迹 大鼠 

分 类 号：R339.3[医药卫生—人体生理学]