咳宁方对实验性豚鼠镇咳平喘作用的药理学研究  

The Pharmacological Study on Antitussive and Antiasthmatic Effect of Kening Fang in Experimantal Guinea Pig

作　　者：杨钦河[1] 凌家生[1] 温承远[1] 彭胜权[2] 平换换[1] 纪桂元[1] 邓丰承[1] 

机构地区：[1]暨南大学医学院中医系,广东广州510632 [2]广州中医药大学,广东广州510405

出　　处：《新中医》2007年第6期104-106,共3页New Chinese Medicine

基　　金：广东省中医药管理局科研基金重点资助项目(编号:97184)

摘　　要：目的:观察咳宁方对实验性豚鼠镇咳、平喘的药理作用。方法:将豚鼠随机分成实验性咳嗽和实验性哮喘各7组,每组10只。实验性咳嗽分为:正常对照组、可待因组、蛇胆川贝液组、咳宁Ⅰ方组(高)、咳宁Ⅰ方组(低)、咳宁Ⅱ方组(高)、咳宁Ⅱ方组(低),以枸橼酸喷雾引咳法,观察豚鼠自喷雾开始到出现咳嗽的潜伏期及喷雾停止后5min内的咳嗽次数。实验性哮喘分组除阳性对照药采用氨茶碱外,其他各组与实验性咳嗽分组相同,用组胺和氯化乙酰胆碱喷雾引喘法,观察豚鼠从喷雾开始到哮喘发作的潜伏期。结果:各用药组咳嗽的潜伏期明显延长,咳嗽次数明显减少,与正常对照组比较,差异均有显著性或非常显著性意义(P＜0.05,P＜0.01),咳宁方的各剂量组与蛇胆川贝液组比较,差异均有显著性或非常显著性意义(P＜0.05,P＜0.01),咳宁Ⅱ方组(低)与咳宁Ⅰ方组(低)比较,差异有显著性意义(P＜0.05)。用药各组豚鼠哮喘潜伏期均有明显延长,与正常对照组比较,差异均有非常显著性意义(P＜0.01),以氨萘碱组最为明显,咳宁Ⅰ方组(高)和咳宁Ⅱ方组(高)与蛇胆川贝液组比较,差异均有显著性意义(P＜0.05)。结论:咳宁方具有明显的镇咳、平喘作用。Objective:To observe the pharmacological action of Kening Fang (KF,咳宁方)for antitusaive and antiasthmatic effect in experimental guinea pig. Methods:The goinea pigs were allocated randomly to 7 groups(10 guinea pigs in each group)with experimental cough(EC)and experimental asthma(EA)individual- ly.The EC was classified to normal control group(NCG),codeine group(CG), Shedan Chuanbei Ye(蛇胆川贝液)group(SCYG),high dose KF I group(HK IG),low dose KF I group(LK IG),high dose KFⅡgroup(HKⅡG)and low dose KFⅡgroup(LKⅡG).Citric acid spraying induction was apphed to induce cough in guinea pigs.The frequency cough from the beginning of spray to incubation period of cough and 5 after suspension of spray were observed.In EA positive controI group was given with aminophylline,the other groups was same as CG.Histamine and acecoline spraying were applied to induce asthma to observe the inbubation period from the be- ginning of spray to the occurrence of asthma.Results:The incubation period of cough in every medicinal group was markedly prolonged and the frequency of cough was markedly decreased,the differences being siguifieant(P＜0.05),P＜0.01)as compared with NCG.The differences between different dose of KF groups and SCYG were significant(P＜0.05,P＜0.01),and that between LK IIG and LK IG was sig- nificant(P＜0.05).The incubatinn period of asthma in various medicinal groups,es- pecially aminophyllin were markedly prolonged(P＜0.01)as compared with NCG, the comparison of HK IG and HK IlG with SCYG showed significant difference(P＜0.05).Conclusion:KF possesses marked antiltussive and antiasthmatic effect.

关 键 词：咳宁方 止咳 平喘 动物 实验 豚鼠 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]