转基因杨树对杨小舟蛾幼虫解毒酶活性的影响  

Effect of Transgenic Poplars on the Activities of Detoxification Enzymes in Micromelalopha troglodyta Larvae

作　　者：郭同斌 嵇保中[2] 诸葛强[2] 黄敏仁[2] 

机构地区：[1]徐州市森林病虫害防治检疫站,徐州221004 [2]南京林业大学森林资源与环境学院,南京210037

出　　处：《林业科学》2007年第5期59-63,共5页Scientia Silvae Sinicae

基　　金：国家研究与开发专项(JY03-B-25);江苏省高新技术资助项目(BG2001313;BG2003306)

摘　　要：用转Bt单基因和转Bt+CpTI双价基因杨树叶片饲喂杨小舟蛾,幼虫中肠酯酶的活力在饲喂初期显著升高,但在饲喂一定时间后受到抑制:转Bt单基因杨树在饲喂24h开始受到抑制,48h受到明显抑制,较对照下降了15.8%;而转双价基因杨树在饲喂12h受到明显抑制,较对照下降了38.1%。转双价基因杨树对中肠酯酶的抑制作用大于转Bt单基因杨树。转Bt单基因杨树对幼虫中肠羧酸酯酶的抑制能力不强,而转双价基因杨树对中肠羧酸酯酶的抑制作用大于转Bt单基因杨树,饲喂12h后活力开始受到抑制,饲喂24、36、48h的活力分别较对照下降了33.4%、22.5%、29.6%,与对照均有显著差异。转基因杨树主要通过抑制幼虫中肠酯酶和羧酸酯酶这2种解毒酶活性而干扰昆虫正常的生理代谢,从而起到毒杀害虫的作用。The larvae of Micromelalopha troglodyta were fed with leaves of transgenic poplars expressing Bt gene only and expressing Bt and CpTI genes, the activities of esterase in the midgut of larvae raised in the earlier stage, but restricted later. When the larvae were treated with leaves of transgenic poplars expressing Bt alone and expressing both genes respectively, the corresponding highest restrictive stage was 48, 12 h, and the activities decreased by 15.8%, 38.1%. The restrictive efficiency of the clone expressing both Bt and CpTI was superior to that expressing Bt alone. The restriction of the poplars with Bt gene to carboxylesterase in the midgut of larvae was not notable, but the plants with Bt and CpTI genes could restrain the activities of the Carboxyleterase significantly. When the larvae were fed with leaves of transgenic poplars expressing both genes for 24, 36, 48 h, the activities of carboxyleterase decreased by 33.4%, 22.5%, 29.6% respectively, which had a significant difference with CK. So it was shown that the transgenic poplars disturbed the insect regular metabolism mainly by restraining the activities of esterase and carboxyleterase in the midgut of larvae, then killed the pests.

关 键 词：转基因杨树 杨小舟蛾 解毒酶 酯酶 羧酸酯酶 

分 类 号：S718.7[农业科学—林学]