北高丛越桔芽器官离体培养与快繁体系的建立  

Rapid Propagation of Plantlets from Sprout of Vaccinium corymbosum in Vitro

作　　者：赵建萍[1] 柏新富[1] 蒋小满[1] 张萍[1] Bi Kehua 

机构地区：[1]鲁东大学生命科学学院,烟台264025 [2]Cansino Agricultural Development Ltd.Langley,V2Y2H8,Canada

出　　处：《林业科学》2007年第5期111-115,F0003,共6页Scientia Silvae Sinicae

摘　　要：The rapid propagation of plantlets of Vaccinium corymbosum in vitro was studied by using the stem-segment with buds as the explant.The optimal culture conditions were set as follows:1)for the initial culture,the explants were sterilized with alcohol,sap nation of cresol and HgCl2,and then cultured on WPM medium containing ZT 3.0 mg·L-1 and 3% sucrose;2)the medium for clump bud induction was WPM + ZT 2.0 mg·L-1 + 3% sugar;3)the medium for robust shoot was WPM + ZT 0～1.0 mg·L-1 + 3% sugar;4)for adventitious root induction from shoots,the medium was 1/2 WPM liquid nutritions containing IBA 1.0～5.0 mg·L-1 and 2% sugar,in which either vermiculite,filter paper or sterilized cotton were added,and shoots were cultured under a photoperiod of 12 h illumination and 12 h darkness alternatively after 14 days in darkness;5)the medium for transplanting was turfy-soil and mosses（V∶V= 1∶1）.The rapid propagation of plantlets of Vaccinium corymbosum in vitro was studied by using the stem-segment with buds as the explant. The optimal culture conditions were set as follows： 1 ） for the initial culture, the explants were sterilized with alcohol, sap nation of cresol and HgC12 , and then cultured on WPM medium containing ZT 3.0 mg· L^- 1 and 3 % sucrose; 2） the medium for clump bud induction was WPM ＋ ZT 2.0 mg·L^- 1 ＋ 3% sugar; 3） the medium for robust shoot was WPM ＋ ZT 0- 1.0 mg·L^- 1 ＋ 3% sugar; 4） for adventitious root induction from shoots, the medium was 1/2 WPM liquid nutritions containing IBA 1.0 - 5.0 mg·L^- 1 and 2 % sugar, in which either vermiculite, filter paper or sterilized cotton were added, and shoots were cultured under a photoperiod of 12 h illumination and 12 h darkness alternatively after 14 days in darkness; 5） the medium for transplanting was tufty-soil and mosses （ V： V = 1 ： 1 ） .

关 键 词：北高丛越桔 芽器官 离体培养 快繁 

分 类 号：S718.46[农业科学—林学] Q943.1[生物学—植物学]